Heat stress affects prostaglandin synthesis in bovine endometrial cells

Shunsuke Sakai, Natsumi Hagihara, Mariko Kuse, Koji Kimura, Kiyoshi Okuda

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Heat stress (HS) negatively affects reproduction in cattle; however, its effect on endocrine function in bovine endometrial cells remains unclear. In this study, we examined the effects of HS on the production of prostaglandin (PG) E2 and PGF2α in the cultured bovine endometrial epithelial and stromal cells separately. To evaluate the effect of HS on endocrine function, the cells were cultured at 38.5°C (control) or 40.5°C (HS). After treatment, PGE2 and PGF2α levels were measured via enzyme immunoassay (EIA) and mRNA expressions of enzymes involved in PG synthesis were examined via quantitative reverse transcription polymerase chain reaction (RT-PCR). HS did not influence the production of PGE2 or PGF2α in the epithelial cells; however, HS significantly enhanced the production of both PGE2 and PGF2α in the stromal cells (P < 0.05). In addition, HS significantly increased phospholipase A2 (PLA2), cyclooxygenase 2 (COX2), prostaglandin F synthase (PGFS), prostaglandin E synthase (PGES), and carbonyl reductase 1 (CBR1) mRNA expression in the stromal cells (P < 0.05). The overall results suggest that HS induces mRNA expression of enzymes involved in PG synthesis, resulting in the upregulation of PGE2 and PGF2α production in the stromal cells, but not in the epithelial cells. The HS-induced increase of PGE2 and PGF2α secretion in bovine endometrial stromal cells may disrupt the normal estrous cycle and cause infertility in cows during summer.

Original languageEnglish
Pages (from-to)311-317
Number of pages7
JournalJournal of Reproduction and Development
Volume64
Issue number4
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

prostaglandins
heat stress
stromal cells
synthesis
cattle
cells
epithelial cells
phospholipase A2
enzyme immunoassays
prostaglandin synthase
enzymes
estrous cycle
quantitative polymerase chain reaction
reverse transcriptase polymerase chain reaction
secretion
cows
summer

Keywords

  • Bovine
  • Endometrial cell
  • Heat stress
  • Prostaglandin

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Heat stress affects prostaglandin synthesis in bovine endometrial cells. / Sakai, Shunsuke; Hagihara, Natsumi; Kuse, Mariko; Kimura, Koji; Okuda, Kiyoshi.

In: Journal of Reproduction and Development, Vol. 64, No. 4, 01.01.2018, p. 311-317.

Research output: Contribution to journalArticle

Sakai, Shunsuke ; Hagihara, Natsumi ; Kuse, Mariko ; Kimura, Koji ; Okuda, Kiyoshi. / Heat stress affects prostaglandin synthesis in bovine endometrial cells. In: Journal of Reproduction and Development. 2018 ; Vol. 64, No. 4. pp. 311-317.
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