Heat stability of Y-chromatin in blood smears

A. Tsutsumi, Y. Yamamoto, H. Ishizu

Research output: Contribution to journalArticle

Abstract

The influence of high temperature on Y chromosome with respect to quinacrine stainings was studied. Blood smears from 5 males and 3 females were tested in this study. Results showed that in male blood Y-chromatins could be detected in more than 10% of nuclei in the leucocytes by quinacrine staining after heating at 100°C for 5h, at 200°C for 60 min and at 300°C for 60s, while in female blood under the same condition Y-chromatins could be detected in 1-2% of nuclei and therefore sex identification could be achieved. This study indicates that Y-chromatin is heat stable.

Original languageEnglish
Pages (from-to)777-782
Number of pages6
JournalJapanese Journal of Legal Medicine
Volume37
Issue number6
Publication statusPublished - Dec 1 1983
Externally publishedYes

ASJC Scopus subject areas

  • Health Policy
  • Law

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