Heat stability of Y-chromatin in blood smears

A. Tsutsumi, Yuji Yamamoto, H. Ishizu

Research output: Contribution to journalArticle

Abstract

The influence of high temperature on Y chromosome with respect to quinacrine stainings was studied. Blood smears from 5 males and 3 females were tested in this study. Results showed that in male blood Y-chromatins could be detected in more than 10% of nuclei in the leucocytes by quinacrine staining after heating at 100°C for 5h, at 200°C for 60 min and at 300°C for 60s, while in female blood under the same condition Y-chromatins could be detected in 1-2% of nuclei and therefore sex identification could be achieved. This study indicates that Y-chromatin is heat stable.

Original languageEnglish
Pages (from-to)777-782
Number of pages6
JournalJapanese Journal of Legal Medicine
Volume37
Issue number6
Publication statusPublished - 1983
Externally publishedYes

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Chromatin
Quinacrine
Hot Temperature
Staining and Labeling
Y Chromosome
Heating
Leukocytes
Temperature

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Heat stability of Y-chromatin in blood smears. / Tsutsumi, A.; Yamamoto, Yuji; Ishizu, H.

In: Japanese Journal of Legal Medicine, Vol. 37, No. 6, 1983, p. 777-782.

Research output: Contribution to journalArticle

Tsutsumi, A, Yamamoto, Y & Ishizu, H 1983, 'Heat stability of Y-chromatin in blood smears', Japanese Journal of Legal Medicine, vol. 37, no. 6, pp. 777-782.
Tsutsumi, A. ; Yamamoto, Yuji ; Ishizu, H. / Heat stability of Y-chromatin in blood smears. In: Japanese Journal of Legal Medicine. 1983 ; Vol. 37, No. 6. pp. 777-782.
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