Heat-shock tagging

A simple method for expression and isolation of plant genome DNA flanked by T-DNA insertions

Shio Matsuhara, Fumito Jingu, Taku Takahashi, Yoshibumi Komeda

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

This paper describes expression profiles of the Arabidopsis HSP18.2 heat-shock gene promoter by using three different reporter genes, and the application of this promoter to a method we have developed to drive heat-shock-dependent transcription of plant genome DNA flanked by T-DNA insertions. We show that, irrespective of the location of the T-DNA insertion, an HSP18.2 promoter towards the left border of the T-DNA effectively induces transcription of flanking genome sequences in response to heat shock. If polyadenylated, tagged transcripts can be easily identified by RT-PCR.

Original languageEnglish
Pages (from-to)79-86
Number of pages8
JournalPlant Journal
Volume22
Issue number1
DOIs
Publication statusPublished - Apr 2000
Externally publishedYes

Fingerprint

Plant DNA
Plant Genome
heat stress
Shock
Hot Temperature
genome
promoter regions
DNA
Heat-Shock Response
transcription (genetics)
Reporter Genes
Arabidopsis
methodology
reporter genes
Genome
Polymerase Chain Reaction
reverse transcriptase polymerase chain reaction
Genes
T-DNA
genes

ASJC Scopus subject areas

  • Plant Science

Cite this

Heat-shock tagging : A simple method for expression and isolation of plant genome DNA flanked by T-DNA insertions. / Matsuhara, Shio; Jingu, Fumito; Takahashi, Taku; Komeda, Yoshibumi.

In: Plant Journal, Vol. 22, No. 1, 04.2000, p. 79-86.

Research output: Contribution to journalArticle

Matsuhara, Shio ; Jingu, Fumito ; Takahashi, Taku ; Komeda, Yoshibumi. / Heat-shock tagging : A simple method for expression and isolation of plant genome DNA flanked by T-DNA insertions. In: Plant Journal. 2000 ; Vol. 22, No. 1. pp. 79-86.
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