Heat-shock tagging: A simple method for expression and isolation of plant genome DNA flanked by T-DNA insertions

Shio Matsuhara, Fumito Jingu, Taku Takahashi, Yoshibumi Komeda

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

This paper describes expression profiles of the Arabidopsis HSP18.2 heat-shock gene promoter by using three different reporter genes, and the application of this promoter to a method we have developed to drive heat-shock-dependent transcription of plant genome DNA flanked by T-DNA insertions. We show that, irrespective of the location of the T-DNA insertion, an HSP18.2 promoter towards the left border of the T-DNA effectively induces transcription of flanking genome sequences in response to heat shock. If polyadenylated, tagged transcripts can be easily identified by RT-PCR.

Original languageEnglish
Pages (from-to)79-86
Number of pages8
JournalPlant Journal
Volume22
Issue number1
DOIs
Publication statusPublished - Apr 2000

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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