Heat shock protein produced by Helicobacter pylori

Kenji Yokota, Y. Hirai, M. Haque, S. Hayashi, H. Isogai, T. Sugiyama, E. Nagamachi, Y. Tsukada, N. Fujii, K. Oguma

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The cells of Helicobacter pylori were suspended in the medium containing 35S-methionine. After a heat shock of the cells at 42 C for 5, 10, and 30 min, the production of proteins was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. Out of many proteins produced by the cells, only 66 kDa protein production was dramatically increased by heat treatment. The N-terminal amino acid sequence of 66 kDa protein was quite similar to that of 62 kDa and 54 kDa proteins previously suggested as heat shock protein (HSP) of H. pylori based on the reaction with polyclonal and monoclonal antibodies against HSP 60 family proteins produced by other bacteria. Therefore, it was concluded that H. pylori produces the 66 kDa protein as its major heat shock protein which belongs to HSP 60 family.

Original languageEnglish
Pages (from-to)403-405
Number of pages3
JournalMicrobiology and Immunology
Volume38
Issue number5
Publication statusPublished - 1994

Fingerprint

Heat-Shock Proteins
Helicobacter pylori
Proteins
Chaperonin 60
Hot Temperature
Autoradiography
Sodium Dodecyl Sulfate
Methionine
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence
Shock
Monoclonal Antibodies
Bacteria

Keywords

  • Heat shock protein
  • Helicobacter pylori
  • Urease

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology
  • Microbiology (medical)

Cite this

Yokota, K., Hirai, Y., Haque, M., Hayashi, S., Isogai, H., Sugiyama, T., ... Oguma, K. (1994). Heat shock protein produced by Helicobacter pylori. Microbiology and Immunology, 38(5), 403-405.

Heat shock protein produced by Helicobacter pylori. / Yokota, Kenji; Hirai, Y.; Haque, M.; Hayashi, S.; Isogai, H.; Sugiyama, T.; Nagamachi, E.; Tsukada, Y.; Fujii, N.; Oguma, K.

In: Microbiology and Immunology, Vol. 38, No. 5, 1994, p. 403-405.

Research output: Contribution to journalArticle

Yokota, K, Hirai, Y, Haque, M, Hayashi, S, Isogai, H, Sugiyama, T, Nagamachi, E, Tsukada, Y, Fujii, N & Oguma, K 1994, 'Heat shock protein produced by Helicobacter pylori', Microbiology and Immunology, vol. 38, no. 5, pp. 403-405.
Yokota K, Hirai Y, Haque M, Hayashi S, Isogai H, Sugiyama T et al. Heat shock protein produced by Helicobacter pylori. Microbiology and Immunology. 1994;38(5):403-405.
Yokota, Kenji ; Hirai, Y. ; Haque, M. ; Hayashi, S. ; Isogai, H. ; Sugiyama, T. ; Nagamachi, E. ; Tsukada, Y. ; Fujii, N. ; Oguma, K. / Heat shock protein produced by Helicobacter pylori. In: Microbiology and Immunology. 1994 ; Vol. 38, No. 5. pp. 403-405.
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