Heat Shock Protein Overexpression-Mediated Periodontal Ligament Regeneration: A Fundamental Approach to Generate a Potential Biomaterial

Rina Muraoka, Keisuke Nakano, Toshiyuki Kawakami

Research output: Contribution to journalArticlepeer-review

Abstract

The periodontal ligament (PDL) is a cell-rich fibrous connective tissue supporting the tooth roots. The tissue helps to maintain homeostasis and exhibits regenerative and repairing ability, which is mediated by the heat shock protein (HSP). Here, we experimentally created PDL tissue with notable ability to regenerate hard tissue and evaluated it as a potential biomaterial. We immunohistochemically examined the mechanical load-induced HSP overexpression in mouse PDL. Following mechanical load application and release, HSP70 localization in the PDL was altered immediately, suggesting that the HSP70 function may differ with the timing of its expression in PDL. HSP70 expressed in the cytoplasm and nucleus of fibroblasts in PDL on the tension side not only participated in periodontium repair, but also functioned as a molecular chaperone during protein expression involved in osteogenesis to restructure injured tissue. This study highlights the potential of artificially created highly functional PDL tissues as biomaterials.

Original languageEnglish
Article number809
JournalMaterials
Volume15
Issue number3
DOIs
Publication statusPublished - Feb 1 2022
Externally publishedYes

Keywords

  • Biomaterials
  • Fibroblasts
  • Heat shock protein (HSP)
  • Homeostasis
  • Mechanical load
  • Periodontal ligament (PDL) tissue
  • Regeneration
  • Remodeling

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics

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