Heat shock factor 1 contributes to Ischemia-induced Angiogenesis by regulating the mobilization and recruitment of bone marrow stem/progenitor cells

Masayuki Kubo, Tao Sheng Li, Hiroshi Kurazumi, Yoshihiro Takemoto, Mako Ohshima, Yumi Yamamoto, Arata Nishimoto, Akihito Mikamo, Mitsuaki Fujimoto, Akira Nakai, Kimikazu Hamano

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Bone marrow (BM)-derived stem/progenitor cells play an important role in ischemia-induced angiogenesis in cardiovascular diseases. Heat shock factor 1 (HSF1) is known to be induced in response to hypoxia and ischemia. We examined whether HSF1 contributes to ischemia-induced angiogenesis through the mobilization and recruitment of BM-derived stem/progenitor cells using HSF1-knockout (KO) mice. After the induction of ischemia, blood flow and microvessel density in the ischemic hindlimb were significantly lower in the HSF1-KO mice than in the wild-type (WT) mice. The mobilization of BM-derived Sca-1- and c-kit-positive cells in peripheral blood after ischemia was significantly lower in the HSF1-KO mice than in the WT mice. BM stem/progenitor cells from HSF1-KO mice showed a significant decrease in their recruitment to ischemic tissue and in migration, adhesion, and survival when compared with WT mice. Blood flow recovery in the ischemic hindlimb significantly decreased in WT mice receiving BM reconstitution with donor cells from HSF1-KO mice. Conversely, blood flow recovery in the ischemic hindlimb significantly increased in HSF1-KO mice receiving BM reconstitution with donor cells from WT mice. These findings suggest that HSF1 contributes to ischemia-induced angiogenesis by regulating the mobilization and recruitment of BM-derived stem/progenitor cells.

Original languageEnglish
Article numbere37934
JournalPloS one
Volume7
Issue number5
DOIs
Publication statusPublished - May 24 2012

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint Dive into the research topics of 'Heat shock factor 1 contributes to Ischemia-induced Angiogenesis by regulating the mobilization and recruitment of bone marrow stem/progenitor cells'. Together they form a unique fingerprint.

  • Cite this

    Kubo, M., Li, T. S., Kurazumi, H., Takemoto, Y., Ohshima, M., Yamamoto, Y., Nishimoto, A., Mikamo, A., Fujimoto, M., Nakai, A., & Hamano, K. (2012). Heat shock factor 1 contributes to Ischemia-induced Angiogenesis by regulating the mobilization and recruitment of bone marrow stem/progenitor cells. PloS one, 7(5), [e37934]. https://doi.org/10.1371/journal.pone.0037934