Heat shock cognate protein 70 contributes to Brucella invasion into trophoblast giant cells that cause infectious abortion

Kenta Watanabe, Masato Tachibana, Satoshi Tanaka, Hidefumi Furuoka, Motohiro Horiuchi, Hiroshi Suzuki, Masahisa Watarai

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background. The cell tropism of Brucella abortus, a causative agent of brucellosis and facultative intracellular pathogen, in the placenta is thought to be a key event of infectious abortion, although the molecular mechanism for this is largely unknown. There is a higher degree of bacterial colonization in the placenta than in other organs and many bacteria are detected in trophoblast giant (TG) cells in the placenta. In the present study, we investigated mechanism of B. abortus invasion into TG cells. Results. We observed internalization and intracellular growth of B. abortus in cultured TG cells. A monoclonal antibody that inhibits bacterial internalization was isolated and this reacted with heat shock cognate protein 70 (Hsc70). Depletion and over expression of Hsc70 in TG cells inhibited and promoted bacterial internalization, respectively. IFN-γ receptor was expressed in TG cells and IFN-γ treatment enhanced the uptake of bacteria by TG cells. Administering the anti-Hsc70 antibody to pregnant mice served to prevent infectious abortion. Conclusion. B. abortus infection of TG cells in placenta is mediated by Hsc70, and that such infection leads to infectious abortion.

Original languageEnglish
Article number212
JournalBMC Microbiology
Volume8
DOIs
Publication statusPublished - 2008

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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