Heat killed multi-serotype Shigella immunogens induced humoral immunity and protection against heterologous challenge in rabbit model

Dhrubajyoti Nag, Ritam Sinha, Soma Mitra, Soumik Barman, Yoshifumi Takeda, Sumio Shinoda, M. K. Chakrabarti, Hemanta Koley

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Recently we have shown the homologous protective efficacy of heat killed multi-serotype Shigella (HKMS) immunogens in a guinea pig colitis model. In our present study, we have advanced our research by immunizing rabbits with a reduced number of oral doses and evaluating the host's adaptive immune responses. The duration of immunogenicity and subsequently protective efficacy was determined against wild type heterologous Shigella strains in a rabbit luminal model. After three successive oral immunizations with HKMS immunogens, serum and lymphocyte supernatant antibody titer against the heterologous shigellae were reciprocally increased and remained at an elevated level up to 180 days. Serogroup and serotype specific O-antigen of lipopolysaccharide and immunogenic proteins of heterologous challenge strains were detected by immunoblot assay. Up-regulation of IL-12p35, IFN-γ and IL-10 mRNA expression was detected in immunized rabbit peripheral blood mononuclear cells (PBMC) after stimulation with HKMS in vitro. HKMS-specific plasma cell response was confirmed by production of a relatively higher level of HKMS-specific IgG in immunized PBMC supernatant compared to control group. Furthermore, the immunized groups of rabbits exhibited complete protection against wild type heterologous shigellae challenge. Thus HKMS immunogens induced humoral and Th1-mediated adaptive immunity and provided complete protection in a rabbit model. These immunogens could be a broad spectrum non-living vaccine candidate for human use in the near future.

Original languageEnglish
Pages (from-to)1275-1283
Number of pages9
JournalImmunobiology
Volume220
Issue number11
DOIs
Publication statusPublished - Nov 1 2015
Externally publishedYes

Fingerprint

Shigella
Humoral Immunity
Hot Temperature
Rabbits
Adaptive Immunity
Blood Cells
Interleukin-12 Subunit p35
Serogroup
O Antigens
Colitis
Plasma Cells
Interleukin-10
Lipopolysaccharides
Immunization
Guinea Pigs
Up-Regulation
Vaccines
Immunoglobulin G
Lymphocytes
Control Groups

Keywords

  • Adaptive immunity
  • Heat killed multi serotype
  • Humoral immunity
  • Rabbit model
  • Shigella

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology

Cite this

Heat killed multi-serotype Shigella immunogens induced humoral immunity and protection against heterologous challenge in rabbit model. / Nag, Dhrubajyoti; Sinha, Ritam; Mitra, Soma; Barman, Soumik; Takeda, Yoshifumi; Shinoda, Sumio; Chakrabarti, M. K.; Koley, Hemanta.

In: Immunobiology, Vol. 220, No. 11, 01.11.2015, p. 1275-1283.

Research output: Contribution to journalArticle

Nag, D, Sinha, R, Mitra, S, Barman, S, Takeda, Y, Shinoda, S, Chakrabarti, MK & Koley, H 2015, 'Heat killed multi-serotype Shigella immunogens induced humoral immunity and protection against heterologous challenge in rabbit model', Immunobiology, vol. 220, no. 11, pp. 1275-1283. https://doi.org/10.1016/j.imbio.2015.07.002
Nag, Dhrubajyoti ; Sinha, Ritam ; Mitra, Soma ; Barman, Soumik ; Takeda, Yoshifumi ; Shinoda, Sumio ; Chakrabarti, M. K. ; Koley, Hemanta. / Heat killed multi-serotype Shigella immunogens induced humoral immunity and protection against heterologous challenge in rabbit model. In: Immunobiology. 2015 ; Vol. 220, No. 11. pp. 1275-1283.
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