Heart slice NMR

Tadayuki Uetani, Daisuke Yamashita, Juichiro Shimizu, Hiromi Misawa, Yasushi Tatematsu, Yukihisa Hamaguchi, Takehiro Miyasaka, Yuki Katanosaka, Toshiaki Kato, Tatsuaki Matsubara, Koichi Furukawa, Toyoaki Murohara, Miyako Takaki, Shinsuke Nakayama

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Nuclear magnetic resonance (NMR) spectroscopy of the heart is normally carried out using whole heart preparations under coronary perfusion. In such preparations, either radical changes in ionic composition of the perfusate or applications of numerous drugs would affect coronary microcirculation. This report communicates the first 31P NMR spectroscopy study using a heart slice preparation (left ventricular slices) superfused with extracellular medium. The ratio of phosphocreatine concentration to ATP concentration was ∼2.1. Also, intracellular pH and Mg2+ concentration ([Mg 2+]i), estimated from the chemical shifts of inorganic phosphate and ATP, were comparable with those undter retrograde perfusion. [Mg2+]i was significantly increased by the removal of extracellular Na+, supporting the essential role of Na +-coupled Mg2+ transport in Mg2+ homeostasis of the heart. Heart slice preparation could also be used to evaluate the potency of cardiac drugs, regardless of their possible effects on coronary microcirculation.

Original languageEnglish
Pages (from-to)H1181-H1186
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number2
Publication statusPublished - Feb 2007


  • Adenosine 5′-triphosphate
  • Heart slice
  • High-energy phosphates
  • Magnesium
  • Nuclear magnetic resonance

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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