Heart slice NMR

Tadayuki Uetani; Daisuke Yamashita; Juichiro Shimizu; Hiromi Misawa; Yasushi Tatematsu; Yukihisa Hamaguchi; Takehiro Miyasaka; Yuki Katanosaka; Toshiaki Kato; Tatsuaki Matsubara; Koichi Furukawa; Toyoaki Murohara; Miyako Takaki; Shinsuke Nakayama

doi:10.1152/ajpheart.00923.2005

Heart slice NMR

Tadayuki Uetani, Daisuke Yamashita, Juichiro Shimizu, Hiromi Misawa, Yasushi Tatematsu, Yukihisa Hamaguchi, Takehiro Miyasaka, Yuki Katanosaka, Toshiaki Kato, Tatsuaki Matsubara, Koichi Furukawa, Toyoaki Murohara, Miyako Takaki, Shinsuke Nakayama

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Nuclear magnetic resonance (NMR) spectroscopy of the heart is normally carried out using whole heart preparations under coronary perfusion. In such preparations, either radical changes in ionic composition of the perfusate or applications of numerous drugs would affect coronary microcirculation. This report communicates the first ₃₁P NMR spectroscopy study using a heart slice preparation (left ventricular slices) superfused with extracellular medium. The ratio of phosphocreatine concentration to ATP concentration was ∼2.1. Also, intracellular pH and Mg²⁺ concentration ([Mg ²⁺]_i), estimated from the chemical shifts of inorganic phosphate and ATP, were comparable with those undter retrograde perfusion. [Mg²⁺]_i was significantly increased by the removal of extracellular Na⁺, supporting the essential role of Na ⁺-coupled Mg²⁺ transport in Mg²⁺ homeostasis of the heart. Heart slice preparation could also be used to evaluate the potency of cardiac drugs, regardless of their possible effects on coronary microcirculation.

Original language	English
Pages (from-to)	H1181-H1186
Journal	American Journal of Physiology - Heart and Circulatory Physiology
Volume	292
Issue number	2
DOIs	https://doi.org/10.1152/ajpheart.00923.2005
Publication status	Published - Feb 2007

Keywords

Adenosine 5′-triphosphate
Heart slice
High-energy phosphates
Magnesium
Nuclear magnetic resonance

ASJC Scopus subject areas

Physiology
Cardiology and Cardiovascular Medicine
Physiology (medical)

Access to Document

10.1152/ajpheart.00923.2005

Cite this

@article{376986a887864032b57853e9bc3a48f2,

title = "Heart slice NMR",

abstract = "Nuclear magnetic resonance (NMR) spectroscopy of the heart is normally carried out using whole heart preparations under coronary perfusion. In such preparations, either radical changes in ionic composition of the perfusate or applications of numerous drugs would affect coronary microcirculation. This report communicates the first 31P NMR spectroscopy study using a heart slice preparation (left ventricular slices) superfused with extracellular medium. The ratio of phosphocreatine concentration to ATP concentration was ∼2.1. Also, intracellular pH and Mg2+ concentration ([Mg 2+]i), estimated from the chemical shifts of inorganic phosphate and ATP, were comparable with those undter retrograde perfusion. [Mg2+]i was significantly increased by the removal of extracellular Na+, supporting the essential role of Na +-coupled Mg2+ transport in Mg2+ homeostasis of the heart. Heart slice preparation could also be used to evaluate the potency of cardiac drugs, regardless of their possible effects on coronary microcirculation.",

keywords = "Adenosine 5′-triphosphate, Heart slice, High-energy phosphates, Magnesium, Nuclear magnetic resonance",

author = "Tadayuki Uetani and Daisuke Yamashita and Juichiro Shimizu and Hiromi Misawa and Yasushi Tatematsu and Yukihisa Hamaguchi and Takehiro Miyasaka and Yuki Katanosaka and Toshiaki Kato and Tatsuaki Matsubara and Koichi Furukawa and Toyoaki Murohara and Miyako Takaki and Shinsuke Nakayama",

year = "2007",

month = feb,

doi = "10.1152/ajpheart.00923.2005",

language = "English",

volume = "292",

pages = "H1181--H1186",

journal = "American Journal of Physiology",

issn = "0002-9513",

publisher = "American Physiological Society",

number = "2",

}

TY - JOUR

T1 - Heart slice NMR

AU - Uetani, Tadayuki

AU - Yamashita, Daisuke

AU - Shimizu, Juichiro

AU - Misawa, Hiromi

AU - Tatematsu, Yasushi

AU - Hamaguchi, Yukihisa

AU - Miyasaka, Takehiro

AU - Katanosaka, Yuki

AU - Kato, Toshiaki

AU - Matsubara, Tatsuaki

AU - Furukawa, Koichi

AU - Murohara, Toyoaki

AU - Takaki, Miyako

AU - Nakayama, Shinsuke

PY - 2007/2

Y1 - 2007/2

N2 - Nuclear magnetic resonance (NMR) spectroscopy of the heart is normally carried out using whole heart preparations under coronary perfusion. In such preparations, either radical changes in ionic composition of the perfusate or applications of numerous drugs would affect coronary microcirculation. This report communicates the first 31P NMR spectroscopy study using a heart slice preparation (left ventricular slices) superfused with extracellular medium. The ratio of phosphocreatine concentration to ATP concentration was ∼2.1. Also, intracellular pH and Mg2+ concentration ([Mg 2+]i), estimated from the chemical shifts of inorganic phosphate and ATP, were comparable with those undter retrograde perfusion. [Mg2+]i was significantly increased by the removal of extracellular Na+, supporting the essential role of Na +-coupled Mg2+ transport in Mg2+ homeostasis of the heart. Heart slice preparation could also be used to evaluate the potency of cardiac drugs, regardless of their possible effects on coronary microcirculation.

AB - Nuclear magnetic resonance (NMR) spectroscopy of the heart is normally carried out using whole heart preparations under coronary perfusion. In such preparations, either radical changes in ionic composition of the perfusate or applications of numerous drugs would affect coronary microcirculation. This report communicates the first 31P NMR spectroscopy study using a heart slice preparation (left ventricular slices) superfused with extracellular medium. The ratio of phosphocreatine concentration to ATP concentration was ∼2.1. Also, intracellular pH and Mg2+ concentration ([Mg 2+]i), estimated from the chemical shifts of inorganic phosphate and ATP, were comparable with those undter retrograde perfusion. [Mg2+]i was significantly increased by the removal of extracellular Na+, supporting the essential role of Na +-coupled Mg2+ transport in Mg2+ homeostasis of the heart. Heart slice preparation could also be used to evaluate the potency of cardiac drugs, regardless of their possible effects on coronary microcirculation.

KW - Adenosine 5′-triphosphate

KW - Heart slice

KW - High-energy phosphates

KW - Magnesium

KW - Nuclear magnetic resonance

UR - http://www.scopus.com/inward/record.url?scp=33846969191&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846969191&partnerID=8YFLogxK

U2 - 10.1152/ajpheart.00923.2005

DO - 10.1152/ajpheart.00923.2005

M3 - Article

C2 - 17040966

AN - SCOPUS:33846969191

VL - 292

SP - H1181-H1186

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0002-9513

IS - 2

ER -

Heart slice NMR

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this