Heart and systemic effects of statin pretreatment in a rat model of abdominal sepsis. Assessment by Tc99m-sestamibi biodistribition

Robson Macedo, Som Mehrbod Javadi, Takahiro Higuchi, Marília Daniela Ferreira De Carvalho, Vanessa De Fátima Lima Paiva Medeiros, Ítalo Medeiros Azevedo, Francisco Pignataro Lima, Aldo Cunha Medeiros

Research output: Contribution to journalArticle

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Abstract

Purpose: To evaluate the heart and the Tc-99m-sestamibi biodistribution after statin pretreatment in a rat model of abdominal sepsis. Methods: Twenty-four Wistar rats were randomly distributed into four groups (n=6 per group): 1) sepsis with simvastatin treatment, 2) sepsis with vehicle, 3) sham control with simvastatin and 4) sham control with vehicle. 24 hours after cecal ligation and puncture rats received 1.0MBq of Tc-99m–sestamibi i.v. 30min after, animals were euthanized for ex-vivo tissue counting and myocardium histological analysis. Results: Myocardial histologic alterations were not detected 24 hours post-sepsis. There was significantly increased cardiac Tc-99m-sestamibi activity in the sepsis group with simvastatin treatment (1.9±0.3%ID/g, p<0.001) in comparison to the sepsis group+vehicle (1.0±0.2%ID/g), control sham group+ simvastatin (1.2±0.3%ID/g) and control sham group (1.3±0.2%ID/g). Significant Tc-99m-sestamibi activity in liver, kidney and lungs was also detected in the sepsis group treated with simvastatinin comparison to the other groups. Conclusions: Statin treatment altered the biodistribution of Tc-99m-sestamibi with increased cardiac and solid organ activity in rats with abdominal sepsis, while no impact on controls. Increased myocardial tracer activity may be a result of a possible protection effect due to increased tissue perfusion mediated by statins.

Original languageEnglish
Pages (from-to)388-393
Number of pages6
JournalActa Cirurgica Brasileira
Volume30
Issue number6
DOIs
Publication statusPublished - Jun 30 2015
Externally publishedYes

Fingerprint

Hydroxymethylglutaryl-CoA Reductase Inhibitors
Sepsis
Simvastatin
Control Groups
Punctures
Ligation
Wistar Rats
Myocardium
Therapeutics
Perfusion
Kidney
Lung
Liver

Keywords

  • Heart
  • Inflammation
  • Rats
  • Sepsis
  • Simvastatin
  • Technetium Tc 99m Sestamibi

ASJC Scopus subject areas

  • Surgery

Cite this

Macedo, R., Javadi, S. M., Higuchi, T., De Carvalho, M. D. F., De Fátima Lima Paiva Medeiros, V., Azevedo, Í. M., ... Medeiros, A. C. (2015). Heart and systemic effects of statin pretreatment in a rat model of abdominal sepsis. Assessment by Tc99m-sestamibi biodistribition. Acta Cirurgica Brasileira, 30(6), 388-393. https://doi.org/10.1590/S0102-865020150060000003

Heart and systemic effects of statin pretreatment in a rat model of abdominal sepsis. Assessment by Tc99m-sestamibi biodistribition. / Macedo, Robson; Javadi, Som Mehrbod; Higuchi, Takahiro; De Carvalho, Marília Daniela Ferreira; De Fátima Lima Paiva Medeiros, Vanessa; Azevedo, Ítalo Medeiros; Lima, Francisco Pignataro; Medeiros, Aldo Cunha.

In: Acta Cirurgica Brasileira, Vol. 30, No. 6, 30.06.2015, p. 388-393.

Research output: Contribution to journalArticle

Macedo, R, Javadi, SM, Higuchi, T, De Carvalho, MDF, De Fátima Lima Paiva Medeiros, V, Azevedo, ÍM, Lima, FP & Medeiros, AC 2015, 'Heart and systemic effects of statin pretreatment in a rat model of abdominal sepsis. Assessment by Tc99m-sestamibi biodistribition', Acta Cirurgica Brasileira, vol. 30, no. 6, pp. 388-393. https://doi.org/10.1590/S0102-865020150060000003
Macedo, Robson ; Javadi, Som Mehrbod ; Higuchi, Takahiro ; De Carvalho, Marília Daniela Ferreira ; De Fátima Lima Paiva Medeiros, Vanessa ; Azevedo, Ítalo Medeiros ; Lima, Francisco Pignataro ; Medeiros, Aldo Cunha. / Heart and systemic effects of statin pretreatment in a rat model of abdominal sepsis. Assessment by Tc99m-sestamibi biodistribition. In: Acta Cirurgica Brasileira. 2015 ; Vol. 30, No. 6. pp. 388-393.
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abstract = "Purpose: To evaluate the heart and the Tc-99m-sestamibi biodistribution after statin pretreatment in a rat model of abdominal sepsis. Methods: Twenty-four Wistar rats were randomly distributed into four groups (n=6 per group): 1) sepsis with simvastatin treatment, 2) sepsis with vehicle, 3) sham control with simvastatin and 4) sham control with vehicle. 24 hours after cecal ligation and puncture rats received 1.0MBq of Tc-99m–sestamibi i.v. 30min after, animals were euthanized for ex-vivo tissue counting and myocardium histological analysis. Results: Myocardial histologic alterations were not detected 24 hours post-sepsis. There was significantly increased cardiac Tc-99m-sestamibi activity in the sepsis group with simvastatin treatment (1.9±0.3{\%}ID/g, p<0.001) in comparison to the sepsis group+vehicle (1.0±0.2{\%}ID/g), control sham group+ simvastatin (1.2±0.3{\%}ID/g) and control sham group (1.3±0.2{\%}ID/g). Significant Tc-99m-sestamibi activity in liver, kidney and lungs was also detected in the sepsis group treated with simvastatinin comparison to the other groups. Conclusions: Statin treatment altered the biodistribution of Tc-99m-sestamibi with increased cardiac and solid organ activity in rats with abdominal sepsis, while no impact on controls. Increased myocardial tracer activity may be a result of a possible protection effect due to increased tissue perfusion mediated by statins.",
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T1 - Heart and systemic effects of statin pretreatment in a rat model of abdominal sepsis. Assessment by Tc99m-sestamibi biodistribition

AU - Macedo, Robson

AU - Javadi, Som Mehrbod

AU - Higuchi, Takahiro

AU - De Carvalho, Marília Daniela Ferreira

AU - De Fátima Lima Paiva Medeiros, Vanessa

AU - Azevedo, Ítalo Medeiros

AU - Lima, Francisco Pignataro

AU - Medeiros, Aldo Cunha

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N2 - Purpose: To evaluate the heart and the Tc-99m-sestamibi biodistribution after statin pretreatment in a rat model of abdominal sepsis. Methods: Twenty-four Wistar rats were randomly distributed into four groups (n=6 per group): 1) sepsis with simvastatin treatment, 2) sepsis with vehicle, 3) sham control with simvastatin and 4) sham control with vehicle. 24 hours after cecal ligation and puncture rats received 1.0MBq of Tc-99m–sestamibi i.v. 30min after, animals were euthanized for ex-vivo tissue counting and myocardium histological analysis. Results: Myocardial histologic alterations were not detected 24 hours post-sepsis. There was significantly increased cardiac Tc-99m-sestamibi activity in the sepsis group with simvastatin treatment (1.9±0.3%ID/g, p<0.001) in comparison to the sepsis group+vehicle (1.0±0.2%ID/g), control sham group+ simvastatin (1.2±0.3%ID/g) and control sham group (1.3±0.2%ID/g). Significant Tc-99m-sestamibi activity in liver, kidney and lungs was also detected in the sepsis group treated with simvastatinin comparison to the other groups. Conclusions: Statin treatment altered the biodistribution of Tc-99m-sestamibi with increased cardiac and solid organ activity in rats with abdominal sepsis, while no impact on controls. Increased myocardial tracer activity may be a result of a possible protection effect due to increased tissue perfusion mediated by statins.

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