Haptoglobin Genotyping by Allele-Specific Polymerase Chain Reaction Amplification

Akemi Yano, Yuji Yamamoto, Satoru Miyaishi, Hideo Ishizu

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

We performed haptoglobin (Hp) genotyping by polymerase chain reaction using allele-specific primer-pairs. The major six genotypes of Hp were identified using this method. Among Japanese individuals living in Ehime and Okayama Prefectures, the allele frequencies were estimated to be Hp2 = 0.723 and HpIS = 0.277. Genotyping of Hp was possible with 0.3ng of DNA and with 0.1 25 μl of blood. It was also possible with whole blood left at room temperature for a month and also with the bloodstains left at room temperature for three years. In the heated blood samples, both alleles, Hp2 and HpIS, were detected in those heated at 100°C for 2h. In bloodstains, Hp2 and HpIS were detected in samples heated at 100°C for 2h and 120°C for 30 min. In addition, the genotype could be detected in samples other than blood such as saliva, hair roots, tissue sections and dental pulps. The present method for Hp genotyping is expected to become a useful method in forensic analysis.

Original languageEnglish
Pages (from-to)173-181
Number of pages9
JournalActa medica Okayama
Volume52
Issue number4
Publication statusPublished - Aug 1 1998

Keywords

  • Allele-specific amplification
  • DNA polymorphism
  • Haptoglobin
  • Personal identification
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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