Abstract
We performed haptoglobin (Hp) genotyping by polymerase chain reaction using allele-specific primer-pairs. The major six genotypes of Hp were identified using this method. Among Japanese individuals living in Ehime and Okayama Prefectures, the allele frequencies were estimated to be Hp2 = 0.723 and HpIS = 0.277. Genotyping of Hp was possible with 0.3ng of DNA and with 0.1 25 μl of blood. It was also possible with whole blood left at room temperature for a month and also with the bloodstains left at room temperature for three years. In the heated blood samples, both alleles, Hp2 and HpIS, were detected in those heated at 100°C for 2h. In bloodstains, Hp2 and HpIS were detected in samples heated at 100°C for 2h and 120°C for 30 min. In addition, the genotype could be detected in samples other than blood such as saliva, hair roots, tissue sections and dental pulps. The present method for Hp genotyping is expected to become a useful method in forensic analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 173-181 |
| Number of pages | 9 |
| Journal | Acta Medica Okayama |
| Volume | 52 |
| Issue number | 4 |
| Publication status | Published - Aug 1998 |
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Keywords
- Allele-specific amplification
- DNA polymorphism
- Haptoglobin
- Personal identification
- Polymerase chain reaction
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
Haptoglobin Genotyping by Allele-Specific Polymerase Chain Reaction Amplification. / Yano, Akemi; Yamamoto, Yuji; Miyaishi, Satoru; Ishizu, Hideo.
In: Acta Medica Okayama, Vol. 52, No. 4, 08.1998, p. 173-181.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Haptoglobin Genotyping by Allele-Specific Polymerase Chain Reaction Amplification
AU - Yano, Akemi
AU - Yamamoto, Yuji
AU - Miyaishi, Satoru
AU - Ishizu, Hideo
PY - 1998/8
Y1 - 1998/8
N2 - We performed haptoglobin (Hp) genotyping by polymerase chain reaction using allele-specific primer-pairs. The major six genotypes of Hp were identified using this method. Among Japanese individuals living in Ehime and Okayama Prefectures, the allele frequencies were estimated to be Hp2 = 0.723 and HpIS = 0.277. Genotyping of Hp was possible with 0.3ng of DNA and with 0.1 25 μl of blood. It was also possible with whole blood left at room temperature for a month and also with the bloodstains left at room temperature for three years. In the heated blood samples, both alleles, Hp2 and HpIS, were detected in those heated at 100°C for 2h. In bloodstains, Hp2 and HpIS were detected in samples heated at 100°C for 2h and 120°C for 30 min. In addition, the genotype could be detected in samples other than blood such as saliva, hair roots, tissue sections and dental pulps. The present method for Hp genotyping is expected to become a useful method in forensic analysis.
AB - We performed haptoglobin (Hp) genotyping by polymerase chain reaction using allele-specific primer-pairs. The major six genotypes of Hp were identified using this method. Among Japanese individuals living in Ehime and Okayama Prefectures, the allele frequencies were estimated to be Hp2 = 0.723 and HpIS = 0.277. Genotyping of Hp was possible with 0.3ng of DNA and with 0.1 25 μl of blood. It was also possible with whole blood left at room temperature for a month and also with the bloodstains left at room temperature for three years. In the heated blood samples, both alleles, Hp2 and HpIS, were detected in those heated at 100°C for 2h. In bloodstains, Hp2 and HpIS were detected in samples heated at 100°C for 2h and 120°C for 30 min. In addition, the genotype could be detected in samples other than blood such as saliva, hair roots, tissue sections and dental pulps. The present method for Hp genotyping is expected to become a useful method in forensic analysis.
KW - Allele-specific amplification
KW - DNA polymorphism
KW - Haptoglobin
KW - Personal identification
KW - Polymerase chain reaction
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M3 - Article
VL - 52
SP - 173
EP - 181
JO - Acta Medica Okayama
JF - Acta Medica Okayama
SN - 0386-300X
IS - 4
ER -