Abstract
Background In this study, we investigated the advantages of fluorescence-guided surgery (FGS) in mice of a portable hand-sized imaging system compared with a large fluorescence imaging system or a long-working-distance fluorescence microscope. Methods Mouse models of human pancreatic cancer for FGS included the following: (1) MiaPaCa-2-expressing green fluorescent protein, (2) BxPC3 labeled with Alexa Fluor 488-conjucated anti-carcinoembryonic antigen (CEA) antibody, and (3) patient-derived orthotopic xenograft (PDOX) labeled with Alexa Fluor 488-conjugated anti-carbohydrate antigen 19-9 antibody. Results Each device could clearly detect the primary MiaPaCa-2-green fluorescent protein tumor and any residual tumor after FGS. In the BxPC3 model labeled with Alexa Fluor 488-conjugated anti-CEA, each device could detect the primary tumor, but the MVX10 could not clearly detect the residual tumor remaining after FGS whereas the other devices could. In the PDOX model labeled with Alexa Fluor 488-conjugated anti-carbohydrate antigen 19-9, only the portable hand-held device could distinguish the residual tumor from the background, and complete resection of the residual tumor was achieved under fluorescence navigation. Conclusions The results described in the present report suggest that the hand-held mobile imaging system can be applied to the clinic for FGS because of its convenient size and high sensitivity which should help make FGS widely used.
Original language | English |
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Pages (from-to) | 510-517 |
Number of pages | 8 |
Journal | Journal of Surgical Research |
Volume | 187 |
Issue number | 2 |
DOIs | |
Publication status | Published - Apr 2014 |
Externally published | Yes |
Keywords
- CA19-9
- CEA
- Cell line
- Fluorescence-guided surgery
- Fluorescent proteins
- In vivo imaging
- Mouse model
- Pancreatic cancer
- Patient-derived orthotopic xenografts (PDOX®)
ASJC Scopus subject areas
- Surgery