Hand-held high-resolution fluorescence imaging system for fluorescence-guided surgery of patient and cell-line pancreatic tumors growing orthotopically in nude mice

Yukihiko Hiroshima, Ali Maawy, Sho Sato, Takashi Murakami, Fuminari Uehara, Shinji Miwa, Shuuya Yano, Masashi Momiyama, Takashi Chishima, Kuniya Tanaka, Michael Bouvet, Itaru Endo, Robert M. Hoffman

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Background In this study, we investigated the advantages of fluorescence-guided surgery (FGS) in mice of a portable hand-sized imaging system compared with a large fluorescence imaging system or a long-working-distance fluorescence microscope. Methods Mouse models of human pancreatic cancer for FGS included the following: (1) MiaPaCa-2-expressing green fluorescent protein, (2) BxPC3 labeled with Alexa Fluor 488-conjucated anti-carcinoembryonic antigen (CEA) antibody, and (3) patient-derived orthotopic xenograft (PDOX) labeled with Alexa Fluor 488-conjugated anti-carbohydrate antigen 19-9 antibody. Results Each device could clearly detect the primary MiaPaCa-2-green fluorescent protein tumor and any residual tumor after FGS. In the BxPC3 model labeled with Alexa Fluor 488-conjugated anti-CEA, each device could detect the primary tumor, but the MVX10 could not clearly detect the residual tumor remaining after FGS whereas the other devices could. In the PDOX model labeled with Alexa Fluor 488-conjugated anti-carbohydrate antigen 19-9, only the portable hand-held device could distinguish the residual tumor from the background, and complete resection of the residual tumor was achieved under fluorescence navigation. Conclusions The results described in the present report suggest that the hand-held mobile imaging system can be applied to the clinic for FGS because of its convenient size and high sensitivity which should help make FGS widely used.

Original languageEnglish
Pages (from-to)510-517
Number of pages8
JournalJournal of Surgical Research
Volume187
Issue number2
DOIs
Publication statusPublished - Apr 1 2014
Externally publishedYes

Fingerprint

Optical Imaging
Tumor Cell Line
Nude Mice
Hand
Fluorescence
Residual Neoplasm
Equipment and Supplies
Carcinoembryonic Antigen
Green Fluorescent Proteins
Heterografts
Carbohydrates
Antigens
Antibodies
Pancreatic Neoplasms
Neoplasms

Keywords

  • CA19-9
  • CEA
  • Cell line
  • Fluorescence-guided surgery
  • Fluorescent proteins
  • In vivo imaging
  • Mouse model
  • Pancreatic cancer
  • Patient-derived orthotopic xenografts (PDOX®)

ASJC Scopus subject areas

  • Surgery

Cite this

Hand-held high-resolution fluorescence imaging system for fluorescence-guided surgery of patient and cell-line pancreatic tumors growing orthotopically in nude mice. / Hiroshima, Yukihiko; Maawy, Ali; Sato, Sho; Murakami, Takashi; Uehara, Fuminari; Miwa, Shinji; Yano, Shuuya; Momiyama, Masashi; Chishima, Takashi; Tanaka, Kuniya; Bouvet, Michael; Endo, Itaru; Hoffman, Robert M.

In: Journal of Surgical Research, Vol. 187, No. 2, 01.04.2014, p. 510-517.

Research output: Contribution to journalArticle

Hiroshima, Y, Maawy, A, Sato, S, Murakami, T, Uehara, F, Miwa, S, Yano, S, Momiyama, M, Chishima, T, Tanaka, K, Bouvet, M, Endo, I & Hoffman, RM 2014, 'Hand-held high-resolution fluorescence imaging system for fluorescence-guided surgery of patient and cell-line pancreatic tumors growing orthotopically in nude mice', Journal of Surgical Research, vol. 187, no. 2, pp. 510-517. https://doi.org/10.1016/j.jss.2013.11.1083
Hiroshima, Yukihiko ; Maawy, Ali ; Sato, Sho ; Murakami, Takashi ; Uehara, Fuminari ; Miwa, Shinji ; Yano, Shuuya ; Momiyama, Masashi ; Chishima, Takashi ; Tanaka, Kuniya ; Bouvet, Michael ; Endo, Itaru ; Hoffman, Robert M. / Hand-held high-resolution fluorescence imaging system for fluorescence-guided surgery of patient and cell-line pancreatic tumors growing orthotopically in nude mice. In: Journal of Surgical Research. 2014 ; Vol. 187, No. 2. pp. 510-517.
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abstract = "Background In this study, we investigated the advantages of fluorescence-guided surgery (FGS) in mice of a portable hand-sized imaging system compared with a large fluorescence imaging system or a long-working-distance fluorescence microscope. Methods Mouse models of human pancreatic cancer for FGS included the following: (1) MiaPaCa-2-expressing green fluorescent protein, (2) BxPC3 labeled with Alexa Fluor 488-conjucated anti-carcinoembryonic antigen (CEA) antibody, and (3) patient-derived orthotopic xenograft (PDOX) labeled with Alexa Fluor 488-conjugated anti-carbohydrate antigen 19-9 antibody. Results Each device could clearly detect the primary MiaPaCa-2-green fluorescent protein tumor and any residual tumor after FGS. In the BxPC3 model labeled with Alexa Fluor 488-conjugated anti-CEA, each device could detect the primary tumor, but the MVX10 could not clearly detect the residual tumor remaining after FGS whereas the other devices could. In the PDOX model labeled with Alexa Fluor 488-conjugated anti-carbohydrate antigen 19-9, only the portable hand-held device could distinguish the residual tumor from the background, and complete resection of the residual tumor was achieved under fluorescence navigation. Conclusions The results described in the present report suggest that the hand-held mobile imaging system can be applied to the clinic for FGS because of its convenient size and high sensitivity which should help make FGS widely used.",
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T1 - Hand-held high-resolution fluorescence imaging system for fluorescence-guided surgery of patient and cell-line pancreatic tumors growing orthotopically in nude mice

AU - Hiroshima, Yukihiko

AU - Maawy, Ali

AU - Sato, Sho

AU - Murakami, Takashi

AU - Uehara, Fuminari

AU - Miwa, Shinji

AU - Yano, Shuuya

AU - Momiyama, Masashi

AU - Chishima, Takashi

AU - Tanaka, Kuniya

AU - Bouvet, Michael

AU - Endo, Itaru

AU - Hoffman, Robert M.

PY - 2014/4/1

Y1 - 2014/4/1

N2 - Background In this study, we investigated the advantages of fluorescence-guided surgery (FGS) in mice of a portable hand-sized imaging system compared with a large fluorescence imaging system or a long-working-distance fluorescence microscope. Methods Mouse models of human pancreatic cancer for FGS included the following: (1) MiaPaCa-2-expressing green fluorescent protein, (2) BxPC3 labeled with Alexa Fluor 488-conjucated anti-carcinoembryonic antigen (CEA) antibody, and (3) patient-derived orthotopic xenograft (PDOX) labeled with Alexa Fluor 488-conjugated anti-carbohydrate antigen 19-9 antibody. Results Each device could clearly detect the primary MiaPaCa-2-green fluorescent protein tumor and any residual tumor after FGS. In the BxPC3 model labeled with Alexa Fluor 488-conjugated anti-CEA, each device could detect the primary tumor, but the MVX10 could not clearly detect the residual tumor remaining after FGS whereas the other devices could. In the PDOX model labeled with Alexa Fluor 488-conjugated anti-carbohydrate antigen 19-9, only the portable hand-held device could distinguish the residual tumor from the background, and complete resection of the residual tumor was achieved under fluorescence navigation. Conclusions The results described in the present report suggest that the hand-held mobile imaging system can be applied to the clinic for FGS because of its convenient size and high sensitivity which should help make FGS widely used.

AB - Background In this study, we investigated the advantages of fluorescence-guided surgery (FGS) in mice of a portable hand-sized imaging system compared with a large fluorescence imaging system or a long-working-distance fluorescence microscope. Methods Mouse models of human pancreatic cancer for FGS included the following: (1) MiaPaCa-2-expressing green fluorescent protein, (2) BxPC3 labeled with Alexa Fluor 488-conjucated anti-carcinoembryonic antigen (CEA) antibody, and (3) patient-derived orthotopic xenograft (PDOX) labeled with Alexa Fluor 488-conjugated anti-carbohydrate antigen 19-9 antibody. Results Each device could clearly detect the primary MiaPaCa-2-green fluorescent protein tumor and any residual tumor after FGS. In the BxPC3 model labeled with Alexa Fluor 488-conjugated anti-CEA, each device could detect the primary tumor, but the MVX10 could not clearly detect the residual tumor remaining after FGS whereas the other devices could. In the PDOX model labeled with Alexa Fluor 488-conjugated anti-carbohydrate antigen 19-9, only the portable hand-held device could distinguish the residual tumor from the background, and complete resection of the residual tumor was achieved under fluorescence navigation. Conclusions The results described in the present report suggest that the hand-held mobile imaging system can be applied to the clinic for FGS because of its convenient size and high sensitivity which should help make FGS widely used.

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