Abstract
Metabolism of 2,5,2',5'-tetrachlorobiphenyl (TCB) was studied using liver microsomes of hamsters and two hamster P450 isoforms, CYP1A2 and 2A8. CYP2A8 catalyzed selectively 4-hydroxylation of 2,5,2',5-TCB at a rate of 21.7 pmol/min/nmol P450. In contrast, CYP1A2 showed no activity for hydroxylation of 2,5,2',5'-TCB. Immunological study revealed that rabbit antiserum against CYP2A8 almost completely inhibited the microsomal 4-hydroxylation but that against CYP1A2 did not. It was also shown that the induction pattern of CYP2A8 protein by P450 inducer was similar to that of the 4-hydroxylase activity in hamster liver microsomes. These results suggest that CYP2A8 plays a major role in the 4-hydroxylation of 2,5,2',5'-TCB in hamster liver.
| Original language | English |
|---|---|
| Pages (from-to) | 685-688 |
| Number of pages | 4 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 225 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 14 1996 |
| Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Fingerprint Dive into the research topics of 'Hamster liver cytochrome P450 (CYP2A8) as a 4-hydroxylase for 2,5,2',5'-tetrachlorobiphenyl'. Together they form a unique fingerprint.
Cite this
Koga, N., Kikuichi, N., Kanamaru, T., Ariyoshi, N., Oguri, K., & Yoshimura, H. (1996). Hamster liver cytochrome P450 (CYP2A8) as a 4-hydroxylase for 2,5,2',5'-tetrachlorobiphenyl. Biochemical and Biophysical Research Communications, 225(2), 685-688. https://doi.org/10.1006/bbrc.1996.1230