Haitian variant Vibrio cholerae O1 strains manifest higher virulence in animal models

Priyanka Ghosh, Ritam Sinha, Prosenjit Samanta, Dhira Rani Saha, Hemanta Koley, Shanta Dutta, Keinosuke Okamoto, Amit Ghosh, T. Ramamurthy, Asish K. Mukhopadhyay

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Vibrio cholerae causes fatal diarrheal disease cholera in humans due to consumption of contaminated water and food. To instigate the disease, the bacterium must evade the host intestinal innate immune system; penetrate the mucus layer of the small intestine, adhere and multiply on the surface of microvilli and produce toxin(s) through the action of virulence associated genes. V. cholerae O1 that has caused a major cholera outbreak in Haiti contained several unique genetic signatures. These novel traits are used to differentiate them from the canonical El Tor strains. Several studies reported the spread of these Haitian variant strains in different parts of the world including Asia and Africa, but there is a paucity of information on the clinical consequence of these genetic changes. To understand the impact of these changes, we undertook a study involving mice and rabbit models to evaluate the pathogenesis. The colonization ability of Haitian variant strain in comparison to canonical El Tor strain was found to be significantly more in both suckling mice and rabbit model. Adult mice also displayed the same results. Besides that, infection patterns of Haitian variant strains showed a completely different picture. Increased mucosal damaging, colonization, and inflammatory changes were observed through hematoxylin-eosin staining and transmission electron microscopy. Fluid accumulation ability was also significantly higher in rabbit model. Our study indicated that these virulence features of the Haitian variant strain may have some association with the severe clinical outcome of the cholera patients in different parts of the world.

Original languageEnglish
Article number111
JournalFrontiers in Microbiology
Volume10
Issue numberFEB
DOIs
Publication statusPublished - Jan 1 2019
Externally publishedYes

Fingerprint

Vibrio cholerae O1
Cholera
Virulence
Animal Models
Rabbits
Haiti
Vibrio cholerae
Mucus
Hematoxylin
Eosine Yellowish-(YS)
Microvilli
Transmission Electron Microscopy
Drinking
Small Intestine
Disease Outbreaks
Immune System
Staining and Labeling
Bacteria
Food
Infection

Keywords

  • Cholera
  • El Tor
  • Haitian variant
  • Pathogenesis
  • V. cholerae

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Ghosh, P., Sinha, R., Samanta, P., Saha, D. R., Koley, H., Dutta, S., ... Mukhopadhyay, A. K. (2019). Haitian variant Vibrio cholerae O1 strains manifest higher virulence in animal models. Frontiers in Microbiology, 10(FEB), [111]. https://doi.org/10.3389/fmicb.2019.00111

Haitian variant Vibrio cholerae O1 strains manifest higher virulence in animal models. / Ghosh, Priyanka; Sinha, Ritam; Samanta, Prosenjit; Saha, Dhira Rani; Koley, Hemanta; Dutta, Shanta; Okamoto, Keinosuke; Ghosh, Amit; Ramamurthy, T.; Mukhopadhyay, Asish K.

In: Frontiers in Microbiology, Vol. 10, No. FEB, 111, 01.01.2019.

Research output: Contribution to journalArticle

Ghosh, P, Sinha, R, Samanta, P, Saha, DR, Koley, H, Dutta, S, Okamoto, K, Ghosh, A, Ramamurthy, T & Mukhopadhyay, AK 2019, 'Haitian variant Vibrio cholerae O1 strains manifest higher virulence in animal models', Frontiers in Microbiology, vol. 10, no. FEB, 111. https://doi.org/10.3389/fmicb.2019.00111
Ghosh, Priyanka ; Sinha, Ritam ; Samanta, Prosenjit ; Saha, Dhira Rani ; Koley, Hemanta ; Dutta, Shanta ; Okamoto, Keinosuke ; Ghosh, Amit ; Ramamurthy, T. ; Mukhopadhyay, Asish K. / Haitian variant Vibrio cholerae O1 strains manifest higher virulence in animal models. In: Frontiers in Microbiology. 2019 ; Vol. 10, No. FEB.
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