Hadamard transform microchip electrophoresis combined with diode laser fluorometry

Kazuki Hata; Yasuhiko Kichise; Takashi Kaneta; Totaro Imasaka

doi:10.1021/ac026330e

Hadamard transform microchip electrophoresis combined with diode laser fluorometry

Kazuki Hata, Yasuhiko Kichise, Takashi Kaneta, Totaro Imasaka

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

The application of a Hadamard transform technique to microchip electrophoresis is described. The sample is electrokinetically injected into a separation channel and is then detected by diode laser-induced fluorometry. The sample and buffer solutions are introduced into the channel by controlling the high voltages applied to the solutions, according to a code determined by a Hadamard matrix. The S/N ratio of the signal in the electropherogram can be improved by a factor of 5 in comparison with that obtained by a conventional single-injection method, although an 8-fold improvement is theoretically predicted when a 255-order matrix is used.

Original language	English
Pages (from-to)	1765-1768
Number of pages	4
Journal	Analytical Chemistry
Volume	75
Issue number	7
DOIs	https://doi.org/10.1021/ac026330e
Publication status	Published - Apr 1 2003
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/ac026330e

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AU - Kaneta, Takashi

AU - Imasaka, Totaro

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