Hadamard transform capillary electrophoresis combined with laser-induced fluorometry using electrokinetic injection

Kazuki Hata, Takashi Kaneta, Totaro Imasaka

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Hadamard transform capillary electrophoresis (HTCE) based on electrokinetic injection allows laser-induced fluorescence detection using a small laser, namely the laser-diode-pumped YAG laser, as an excitation source. A small hole is fabricated at the center of a capillary by laser ablation; this hole functions as an inlet port for a sample solution. Therefore, the sample solution can be introduced electrophoretically into the capillary through the small hole. Multiple sample injection is accomplished by introducing a buffer solution from the end of the capillary and the sample solution through the hole. Both solutions are injected using two sets of high-voltage power supplies and migrate toward the opposite end of the capillary. A fluorescent analyte, rhodamine B, is successfully detected in the case of both single and multiple injection according to the Hadamard sequence code. By transforming the data encoded by the Hadamard matrix, the decoded data showed an increase in the signal-to-noise (S/N) ratio by a factor of 9.8. In the case of the sample containing two amino acids labeled with rhodamine B isothiocyanate (RBITC), although the concentration of every component including free RBITC is lower than the concentration limit of detection obtained by single injection, a substantial improvement in the sensitivity is achieved and all components are identified by the Hadamard transform technique.

Original languageEnglish
Pages (from-to)178-182
Number of pages5
JournalAnalytica Chimica Acta
Volume556
Issue number1
DOIs
Publication statusPublished - Jan 18 2006
Externally publishedYes

Fingerprint

Hadamard transforms
fluorometry
Capillary electrophoresis
Fluorometry
Capillary Electrophoresis
electrokinesis
Lasers
transform
laser
Injections
rhodamine B
laser induced fluorescence
Hadamard matrices
Injection lasers
Electric Power Supplies
Semiconductor Lasers
ablation
signal-to-noise ratio
Solid-State Lasers
Laser Therapy

Keywords

  • Amino acid
  • Capillary electrophoresis
  • Hadamard transformation
  • Injection device
  • Laser-induced fluorometry
  • Sensitive detection

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Hadamard transform capillary electrophoresis combined with laser-induced fluorometry using electrokinetic injection. / Hata, Kazuki; Kaneta, Takashi; Imasaka, Totaro.

In: Analytica Chimica Acta, Vol. 556, No. 1, 18.01.2006, p. 178-182.

Research output: Contribution to journalArticle

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