H. pylori decreases gastric mucin synthesis via inhibition of galactosyltransferase

Shouichi Tanaka, Motowo Mizuno, Toshirou Maga, Fumiya Yoshinaga, Jun Tomoda, Junichirou Nasu, Hiroyuki Okada, Kenji Yokota, Keiji Oguma, Yasushi Shiratori, Takao Tsuji

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background/Aims: Alterations of gastric mucin have been postulated as important pathogenic properties of Helicobacter pylori. In this study, we investigated gastric mucin synthesis in H. pylori-infected gastric mucosa by measuring UDP-galactosyltransferase activity, a key enzyme for the synthesis of mucin, and the amount of intracellular mucin in the gastric mucosa. Methodology: Gastric biopsy specimens were obtained from thirty-seven patients (20 H. pylori-positive and 17 H. pylori-negative). UDP-galactosyl-transferase activity of the biopsy specimens was measured by an assay system we had developed, using a peanut agglutinin lectin. The amount of intracellular mucin in the gastric epithelial cells was analyzed by measuring the cells' periodic acid-Schiff-alcian blue staining-positive substances. Results: UDP-galactosyltransferase activities in the antral mucosa, but not in the body mucosa, of H. pylori-positive patients were significantly lower than those of H. pylori-negative patients (p

Original languageEnglish
Pages (from-to)1739-1742
Number of pages4
JournalHepato-Gastroenterology
Volume50
Issue number53
Publication statusPublished - Sep 2003

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Gastric Mucins
Galactosyltransferases
Pylorus
Helicobacter pylori
Uridine Diphosphate
Peanut Agglutinin
Mucins
Gastric Mucosa
Mucous Membrane
Biopsy
Alcian Blue
Periodic Acid
Transferases
Stomach
Epithelial Cells
Staining and Labeling
Enzymes

Keywords

  • Helicobacter pylori
  • Mucin
  • UDP-galactosyltransferase

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Tanaka, S., Mizuno, M., Maga, T., Yoshinaga, F., Tomoda, J., Nasu, J., ... Tsuji, T. (2003). H. pylori decreases gastric mucin synthesis via inhibition of galactosyltransferase. Hepato-Gastroenterology, 50(53), 1739-1742.

H. pylori decreases gastric mucin synthesis via inhibition of galactosyltransferase. / Tanaka, Shouichi; Mizuno, Motowo; Maga, Toshirou; Yoshinaga, Fumiya; Tomoda, Jun; Nasu, Junichirou; Okada, Hiroyuki; Yokota, Kenji; Oguma, Keiji; Shiratori, Yasushi; Tsuji, Takao.

In: Hepato-Gastroenterology, Vol. 50, No. 53, 09.2003, p. 1739-1742.

Research output: Contribution to journalArticle

Tanaka, S, Mizuno, M, Maga, T, Yoshinaga, F, Tomoda, J, Nasu, J, Okada, H, Yokota, K, Oguma, K, Shiratori, Y & Tsuji, T 2003, 'H. pylori decreases gastric mucin synthesis via inhibition of galactosyltransferase', Hepato-Gastroenterology, vol. 50, no. 53, pp. 1739-1742.
Tanaka S, Mizuno M, Maga T, Yoshinaga F, Tomoda J, Nasu J et al. H. pylori decreases gastric mucin synthesis via inhibition of galactosyltransferase. Hepato-Gastroenterology. 2003 Sep;50(53):1739-1742.
Tanaka, Shouichi ; Mizuno, Motowo ; Maga, Toshirou ; Yoshinaga, Fumiya ; Tomoda, Jun ; Nasu, Junichirou ; Okada, Hiroyuki ; Yokota, Kenji ; Oguma, Keiji ; Shiratori, Yasushi ; Tsuji, Takao. / H. pylori decreases gastric mucin synthesis via inhibition of galactosyltransferase. In: Hepato-Gastroenterology. 2003 ; Vol. 50, No. 53. pp. 1739-1742.
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