H. pylori decreases gastric mucin synthesis via inhibition of galactosyltransferase

Shouichi Tanaka, Motowo Mizuno, Toshirou Maga, Fumiya Yoshinaga, Jun Tomoda, Junichirou Nasu, Hiroyuki Okada, Kenji Yokota, Keiji Oguma, Yasushi Shiratori, Takao Tsuji

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23 Citations (Scopus)

Abstract

Background/Aims: Alterations of gastric mucin have been postulated as important pathogenic properties of Helicobacter pylori. In this study, we investigated gastric mucin synthesis in H. pylori-infected gastric mucosa by measuring UDP-galactosyltransferase activity, a key enzyme for the synthesis of mucin, and the amount of intracellular mucin in the gastric mucosa. Methodology: Gastric biopsy specimens were obtained from thirty-seven patients (20 H. pylori-positive and 17 H. pylori-negative). UDP-galactosyl-transferase activity of the biopsy specimens was measured by an assay system we had developed, using a peanut agglutinin lectin. The amount of intracellular mucin in the gastric epithelial cells was analyzed by measuring the cells' periodic acid-Schiff-alcian blue staining-positive substances. Results: UDP-galactosyltransferase activities in the antral mucosa, but not in the body mucosa, of H. pylori-positive patients were significantly lower than those of H. pylori-negative patients (p<0.05). The amount of intracellular mucin in antral epithelial cells of H. pylori-positive patients was significantly lower than that of H. pylori-negative patients (p<0.01). Conclusions: These findings suggest that H. pylori infection decreases gastric mucin synthesis via inhibition of UDP-galactosyltransferase. This effect may impair the gastric mucosal barrier and contribute to the mucosal injury induced by H. pylori infection.

Original languageEnglish
Pages (from-to)1739-1742
Number of pages4
JournalHepato-gastroenterology
Volume50
Issue number53
Publication statusPublished - Sep 2003

Keywords

  • Helicobacter pylori
  • Mucin
  • UDP-galactosyltransferase

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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    Tanaka, S., Mizuno, M., Maga, T., Yoshinaga, F., Tomoda, J., Nasu, J., Okada, H., Yokota, K., Oguma, K., Shiratori, Y., & Tsuji, T. (2003). H. pylori decreases gastric mucin synthesis via inhibition of galactosyltransferase. Hepato-gastroenterology, 50(53), 1739-1742.