H 2O 2 production by copper amine oxidase, a component of the ecto-apyrase (ATPase)-containing protein complex(es) in the pea cell wall, is regulated by an elicitor and a suppressor from Mycosphaerella pinodes

Kazuhiro Toyoda, Eri Yasunaga, Masanobu Niwa, Yuko Ohwatari, Atsushi Nakashima, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Blue native PAGE analysis for cell wall proteins from pea epicotyls demonstrated that cell wall-associated ecto-apyrase (ATPase) formed a large protein complex(es) ranging from 450 to 900 kDa; one of the components of the complex was copper amine oxidase (CuAO), which catalyzes the oxidation of amines with the subsequent generation of ammonia and hydrogen peroxide. CuAO activity was coordinately regulated in vitro with ATP-hydrolyzing activity by an elicitor and a suppressor from Mycosphaerella pinodes. Moreover, treatment of cell wall proteins with the suppressor caused the appearance of the apyrase monomer. On the basis of these results, M. pinodes may target the apyrase-containing protein complex(es) of the host to attenuate cell wall-based, extracellular defense(s) including the production of hydrogen peroxide.

Original languageEnglish
Pages (from-to)311-315
Number of pages5
JournalJournal of General Plant Pathology
Volume78
Issue number5
DOIs
Publication statusPublished - Sep 2012

Fingerprint

Mycosphaerella pinodes
apyrase
amines
adenosinetriphosphatase
peas
copper
cell walls
hydrogen peroxide
proteins
epicotyls
ammonia
oxidation
elicitors

Keywords

  • Blue native PAGE
  • Cell wall-associated ecto-apyrase (ATPase)
  • Copper amine oxidase
  • Mycosphaerella pinodes
  • Pea (Pisum sativum L.)
  • Suppressor

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

H 2O 2 production by copper amine oxidase, a component of the ecto-apyrase (ATPase)-containing protein complex(es) in the pea cell wall, is regulated by an elicitor and a suppressor from Mycosphaerella pinodes. / Toyoda, Kazuhiro; Yasunaga, Eri; Niwa, Masanobu; Ohwatari, Yuko; Nakashima, Atsushi; Inagaki, Yoshishige; Ichinose, Yuki; Shiraishi, Tomonori.

In: Journal of General Plant Pathology, Vol. 78, No. 5, 09.2012, p. 311-315.

Research output: Contribution to journalArticle

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