TY - JOUR
T1 - Gymnema sylvestre Leaf Extract
T2 - A 52-Week Dietary Toxicity Study in Wistar Rats
AU - Ogawa, Yukio
AU - Sekita, Kiyoshi
AU - Umemura, Takashi
AU - Saito, Minoru
AU - Ono, Atsushi
AU - Kawasaki, Yasushi
AU - Uchida, Osayuki
AU - Matsushima, Yuko
AU - Inoue, Tohru
AU - Kanno, Jun
PY - 2004
Y1 - 2004
N2 - A 52-week study of oral-repeated-dose toxicity for the extraction powder of Gymnema sylvestre (GS), Indian-native genus, Metaplexis japonica, was conducted in both genders of Wistar rats. The rats were administered a graded dose of GS at 0.01, 0.10 and 1.00% of basal powder diet, along with a group fed solely with the basal powder diet without GS, for 52 weeks. General conditions were recorded daily. Body weights and food consumptions were recorded weekly up to 12 weeks, and thereafter at longer intervals. At 26 weeks, for an intermediate examination, and 52 weeks, for the final examination, animals were subjected to hematology, serum chemistry, and pathological examination. None of the animals died in the period up to 52 weeks. No exposure-related changes in body-weight, in the food consumption, in the hematological examinations, or in the serum biochemical examinations were recognized. No histopathological alterations were seen. Thus, it was concluded that there was no toxic effect in rats treated with GS at up to 1.00% in the diet for 52 weeks. The no-observable-effect level from this study is 1.00% GS, ie., 504 mg/kg/day for male and 563 mg/kg/day for female as mean daily intake, for 52 weeks.
AB - A 52-week study of oral-repeated-dose toxicity for the extraction powder of Gymnema sylvestre (GS), Indian-native genus, Metaplexis japonica, was conducted in both genders of Wistar rats. The rats were administered a graded dose of GS at 0.01, 0.10 and 1.00% of basal powder diet, along with a group fed solely with the basal powder diet without GS, for 52 weeks. General conditions were recorded daily. Body weights and food consumptions were recorded weekly up to 12 weeks, and thereafter at longer intervals. At 26 weeks, for an intermediate examination, and 52 weeks, for the final examination, animals were subjected to hematology, serum chemistry, and pathological examination. None of the animals died in the period up to 52 weeks. No exposure-related changes in body-weight, in the food consumption, in the hematological examinations, or in the serum biochemical examinations were recognized. No histopathological alterations were seen. Thus, it was concluded that there was no toxic effect in rats treated with GS at up to 1.00% in the diet for 52 weeks. The no-observable-effect level from this study is 1.00% GS, ie., 504 mg/kg/day for male and 563 mg/kg/day for female as mean daily intake, for 52 weeks.
KW - 52-week toxicity study
KW - Gymnema sylvestre leaf extract
KW - Health food
UR - http://www.scopus.com/inward/record.url?scp=12144290784&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=12144290784&partnerID=8YFLogxK
U2 - 10.3358/shokueishi.45.8
DO - 10.3358/shokueishi.45.8
M3 - Article
C2 - 15168555
AN - SCOPUS:12144290784
VL - 45
SP - 8
EP - 18
JO - Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan
JF - Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan
SN - 0015-6426
IS - 1
ER -
