Gut microbiota of healthy and malnourished children in Bangladesh

Shirajum Monira, Shota Nakamura, Kazuyoshi Goto, Kaori Izutsu, Haruo Watanabe, Nur Haque Alam, Hubert Ph Endtz, Alejandro Cravioto, Sk Imran Ali, Takaaki Nakaya, Toshihiro Horii, Tetsuya Iida, Munirul Alam

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Poor health and malnutrition in preschool children are longstanding problems in Bangladesh. Gut microbiota plays a tremendous role in nutrient absorption and determining the state of health. In this study, metagenomic tool was employed to assess the gut microbiota composition of healthy and malnourished children. DNA was extracted from fecal samples of seven healthy and seven malnourished children (n= 14; age 2-3years) were analyzed for the variable region of 16S rRNA genes by universal primer PCR followed by high-throughput 454 parallel sequencing to identify the bacterial phyla and genera. Our results reveal that the healthy children had a significantly higher number of operational taxonomic unit in their gut than that of the malnourished children (healthy vs. malnourished: 546 vs. 310). In malnourished children, bacterial population of the phyla Proteobacteria and Bacteroidetes accounted for 46 and 18%, respectively. Conversely, in healthy children, Proteobacteria and Bacteroidetes accounted for 5% and 44, respectively (p <0.001). In malnourished children, the phylum Proteobacteria included pathogenic genera, namely Klebsiella and Escherichia, which were 174-fold and 9-fold higher, respectively, than their healthy counterpart. The predominance of potentially pathogenic Proteobacteria and minimal level of Bacteroidetes as commensal microbiota might be associated to the ill health of malnourished children in Bangladesh.

Original languageEnglish
JournalFrontiers in Microbiology
Volume2
Issue numberNOV
DOIs
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

Bangladesh
Proteobacteria
Bacteroidetes
Child Nutrition Disorders
Escherichia
Metagenomics
Klebsiella
Microbiota
Health
Preschool Children
Gastrointestinal Microbiome
rRNA Genes
Food
Polymerase Chain Reaction
DNA
Population

Keywords

  • 16S rDNA
  • Children
  • Gut
  • Microbiota
  • Nutrition

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Monira, S., Nakamura, S., Goto, K., Izutsu, K., Watanabe, H., Alam, N. H., ... Alam, M. (2011). Gut microbiota of healthy and malnourished children in Bangladesh. Frontiers in Microbiology, 2(NOV). https://doi.org/10.3389/fmicb.2011.00228

Gut microbiota of healthy and malnourished children in Bangladesh. / Monira, Shirajum; Nakamura, Shota; Goto, Kazuyoshi; Izutsu, Kaori; Watanabe, Haruo; Alam, Nur Haque; Endtz, Hubert Ph; Cravioto, Alejandro; Ali, Sk Imran; Nakaya, Takaaki; Horii, Toshihiro; Iida, Tetsuya; Alam, Munirul.

In: Frontiers in Microbiology, Vol. 2, No. NOV, 2011.

Research output: Contribution to journalArticle

Monira, S, Nakamura, S, Goto, K, Izutsu, K, Watanabe, H, Alam, NH, Endtz, HP, Cravioto, A, Ali, SI, Nakaya, T, Horii, T, Iida, T & Alam, M 2011, 'Gut microbiota of healthy and malnourished children in Bangladesh', Frontiers in Microbiology, vol. 2, no. NOV. https://doi.org/10.3389/fmicb.2011.00228
Monira, Shirajum ; Nakamura, Shota ; Goto, Kazuyoshi ; Izutsu, Kaori ; Watanabe, Haruo ; Alam, Nur Haque ; Endtz, Hubert Ph ; Cravioto, Alejandro ; Ali, Sk Imran ; Nakaya, Takaaki ; Horii, Toshihiro ; Iida, Tetsuya ; Alam, Munirul. / Gut microbiota of healthy and malnourished children in Bangladesh. In: Frontiers in Microbiology. 2011 ; Vol. 2, No. NOV.
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