Growth inhibition and differentiation of cultured smooth muscle cells depend on cellular crossbridges across the tubular lumen of type I collagen matrix honeycombs

Takaaki Suzuki, Itsuko Ishii, Akira Kotani, Michi Masuda, Kaori Hirata, Madoka Ueda, Takahiro Ogata, Takanori Sakai, Noritaka Ariyoshi, Mitsukazu Kitada

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Although rabbit vascular smooth muscle cells (SMCs) showed a differentiated phenotype in three-dimensional type I collagen matrices (honeycombs, diameter of pores = 200-500 μm), mouse vascular SMCs proliferated in honeycombs having the same pore size. Here we investigated the relationship between pore sizes of honeycombs and differentiation of SMCs using various pore sizes of honeycombs. Rabbit SMCs (length: 200 ± 32 μm) and mouse SMCs (49 ± 10 μm) formed crossbridges in honeycombs with 200-300 μm and less than 200 μm of pores, respectively. Both SMCs spread on the inner wall but did not form crossbridges in honeycombs with larger pores. [3H]Thymidine incorporation and cell number of both SMCs were decreased when the crossbridges were formed in honeycombs. Because proliferation inhibition and crossbridge formation were observed in the culture of rabbit and mouse SMCs using 200-300 μm and less than 200 μm pore sized honeycombs, respectively, these data suggested that forming crossbridges was important for the inhibition of proliferation of SMCs. Rabbit SMCs differentiation was accompanied by the expression of caldesmon heavy chain when cultured in honeycombs having less than 300 μm pores. Proliferation of mouse SMCs stopped in honeycombs having less than 200 μm pores, but caldesmon heavy chain was not detected despite the expression of its mRNA. Proliferation of SMCs stopped on plates when cells reached confluent state, however, caldesmon heavy chain was not expressed. These data suggested that an appropriate structure and suitable honeycomb pore size are important for the differentiation of SMCs.

Original languageEnglish
Pages (from-to)143-149
Number of pages7
JournalMicrovascular Research
Volume77
Issue number2
DOIs
Publication statusPublished - Mar 2009
Externally publishedYes

Fingerprint

Collagen Type I
Smooth Muscle Myocytes
Muscle
Cells
Growth
Calmodulin-Binding Proteins
Pore size
Rabbits
Vascular Smooth Muscle
Thymidine
Cell Differentiation
Cell Count

Keywords

  • Crossbridges
  • Differentiation
  • Proliferation
  • Smooth muscle cell
  • Three-dimensional culture

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

Cite this

Growth inhibition and differentiation of cultured smooth muscle cells depend on cellular crossbridges across the tubular lumen of type I collagen matrix honeycombs. / Suzuki, Takaaki; Ishii, Itsuko; Kotani, Akira; Masuda, Michi; Hirata, Kaori; Ueda, Madoka; Ogata, Takahiro; Sakai, Takanori; Ariyoshi, Noritaka; Kitada, Mitsukazu.

In: Microvascular Research, Vol. 77, No. 2, 03.2009, p. 143-149.

Research output: Contribution to journalArticle

Suzuki, Takaaki ; Ishii, Itsuko ; Kotani, Akira ; Masuda, Michi ; Hirata, Kaori ; Ueda, Madoka ; Ogata, Takahiro ; Sakai, Takanori ; Ariyoshi, Noritaka ; Kitada, Mitsukazu. / Growth inhibition and differentiation of cultured smooth muscle cells depend on cellular crossbridges across the tubular lumen of type I collagen matrix honeycombs. In: Microvascular Research. 2009 ; Vol. 77, No. 2. pp. 143-149.
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