Growth behavior of Aβ protofibrils on liposome membranes and their membrane perturbation effect

Toshinori Shimanouchi, Keiichi Nishiyama, Azusa Hiroiwa, Huong Thi Vu, Nachi Kitaura, Hiroshi Umakoshi, Ryoichi Kuboi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The protofibrils of amyloid fibrils have recently drawn an attention due to their cytotoxicity although the physicochemical properties of the protofibrils as an intermediate of fibrillation process have still remained unclear. We investigated the growth behavior of the protofibrils of amyloid β protein with 40 amino acid residues in the presence of various liposomes and the membrane perturbation (calcein leakage). The growth behavior of protofibrils was apparently correlated with the protofibrils-induced calcein leakage. From the binding experiment using a hydrophobic fluorescence probe and the quartz crystal microbalance method combined with the planar lipid membrane, it is suggested that the apparent correlation between the growth behavior of protofibrils and their membrane perturbation resulted from the physicochemical properties of protofibrils such as hydrophobicity and the stability of hydrogen bonds. The protofibrils were found to show the highest hydrophobicity and the highest instability of hydrogen bonds of the proteins used in this study (monomer, protofibrils, matured fibrils, and other three typical proteins). It is anticipated that such properties of protofibrils were advantageous both for the binding of protofibrils with monomer (growth behavior) and for the binding of lipid bilayer membranes (membrane perturbation).

Original languageEnglish
Pages (from-to)81-88
Number of pages8
JournalBiochemical Engineering Journal
Volume71
DOIs
Publication statusPublished - Feb 5 2013
Externally publishedYes

Fingerprint

Liposomes
Membranes
Hydrophobicity
Membrane Lipids
Growth
Hydrophobic and Hydrophilic Interactions
Hydrogen
Hydrogen bonds
Monomers
Quartz Crystal Microbalance Techniques
Proteins
Amyloidogenic Proteins
Lipid bilayers
Quartz crystal microbalances
Lipid Bilayers
Cytotoxicity
Amyloid
Amino acids
Fluorescence
Amino Acids

Keywords

  • Amyloid
  • Hydrogen bonds
  • Hydrophobicity
  • Liposome
  • Protofibril

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Environmental Engineering

Cite this

Growth behavior of Aβ protofibrils on liposome membranes and their membrane perturbation effect. / Shimanouchi, Toshinori; Nishiyama, Keiichi; Hiroiwa, Azusa; Vu, Huong Thi; Kitaura, Nachi; Umakoshi, Hiroshi; Kuboi, Ryoichi.

In: Biochemical Engineering Journal, Vol. 71, 05.02.2013, p. 81-88.

Research output: Contribution to journalArticle

Shimanouchi, Toshinori ; Nishiyama, Keiichi ; Hiroiwa, Azusa ; Vu, Huong Thi ; Kitaura, Nachi ; Umakoshi, Hiroshi ; Kuboi, Ryoichi. / Growth behavior of Aβ protofibrils on liposome membranes and their membrane perturbation effect. In: Biochemical Engineering Journal. 2013 ; Vol. 71. pp. 81-88.
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