Growth arrest of vascular smooth muscle cells in suspension culture using low-acyl gellan gum

Tomomi Natori, Masachika Fujiyoshi, Masashi Uchida, Natsuki Abe, Tatsuro Kanaki, Yasunori Fukumoto, Itsuko Ishii

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The proliferation of vascular smooth muscle cells (SMCs) causes restenosis in biomaterial vascular grafts. The purposes of this study were to establish a suspension culture system for SMCs by using a novel substrate, low-acyl gellan gum (GG) and to maintain SMCs in a state of growth inhibition. When SMCs were cultured in suspension with GG, their proliferation was inhibited. Their viability was 70% at day 2, which was maintained at more than 50% until day 5. In contrast, the viability of cells cultured in suspension without GG was 5.6% at day 2. By cell cycle analysis, the ratio of SMCs in the S phase when cultured in suspension with GG was lower than when cultured on plastic plates. In SMCs cultured in suspension with GG, the ratio of phosphorylated retinoblastoma (Rb) protein to Rb protein was decreased and p27Kip1 expression was unchanged in comparison with SMCs cultured on plastic plates. In addition, SMCs could be induced to proliferate again by changing the culture condition from suspension with GG to plastic plates. These results suggest that our established culturing method for SMCs is useful to maintain SMCs in a state of growth inhibition with high viability.

Original languageEnglish
Pages (from-to)191-198
Number of pages8
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume53
Issue number3
DOIs
Publication statusPublished - Mar 1 2017

Keywords

  • Low-acyl gellan gum
  • Polysaccharide
  • Vascular smooth muscle cells
  • p27

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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