Green Synthesis of Gold Nanoparticles Coupled with Nucleic Acid Oxidation

Tatsuki Kunoh, Minoru Takeda, Syuji Matsumoto, Ichiro Suzuki, Mikio Takano, Hitoshi Kunoh, Jun Takada

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Green synthesis of metal nanoparticles, especially gold nanoparticles (AuNPs), has attracted the great interest of scientists and engineers in the medical and pharmaceutical fields; thus, a variety of ecofriendly, energy- and cost-saving techniques have been developed. In this study, we found that cells of Leptothrix (iron-oxidizing bacteria) released extracellular RNA some of which could exist as a constituent of the cell-enclosing sheaths. As a part of studies of metal encrustation in the sheaths, here we show that RNA prepared from the Leptothrix cells can reduce Au(III) and spherical AuNPs eventually form when an aqueous HAuCl4 solution is added under ambient conditions. RNA and DNA of other organismal origins have the same ability. Of the nucleosides and nucleobases, only guanosine and guanine can form AuNPs. The DNA moiety, 2′-deoxyguanosine (dG) (used as a reference material), forms AuNPs when mixed with HAuCl4 solution, but 8-hydroxy-2′-deoxyguanosine (8-OHdG) does not, indicating that AuNP formation evidently depends on the reduction potential of the guanine moiety, not the sugar moiety. This finding is the first demonstration that spherical AuNPs of ca. 5 nm diameter can be obtained by simply adding guanine to HAuCl4 solution at ambient temperature; no other chemicals or physical treatments are needed.

Original languageEnglish
Pages (from-to)364-373
Number of pages10
JournalACS Sustainable Chemistry and Engineering
Volume6
Issue number1
DOIs
Publication statusPublished - Jan 2 2018

Fingerprint

Nucleic acids
Guanine
nucleic acid
RNA
Gold
Nucleic Acids
gold
Nanoparticles
oxidation
Oxidation
DNA
material form
encrustation
Deoxyguanosine
Guanosine
Metal nanoparticles
metal
Nucleosides
Sugars
Drug products

Keywords

  • 8-OHdG
  • Green synthesis of AuNPs
  • Guanine
  • Nucleic acid oxidation
  • Nucleic acids
  • RNA in Leptothrix sheaths

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Renewable Energy, Sustainability and the Environment

Cite this

Kunoh, T., Takeda, M., Matsumoto, S., Suzuki, I., Takano, M., Kunoh, H., & Takada, J. (2018). Green Synthesis of Gold Nanoparticles Coupled with Nucleic Acid Oxidation. ACS Sustainable Chemistry and Engineering, 6(1), 364-373. https://doi.org/10.1021/acssuschemeng.7b02610

Green Synthesis of Gold Nanoparticles Coupled with Nucleic Acid Oxidation. / Kunoh, Tatsuki; Takeda, Minoru; Matsumoto, Syuji; Suzuki, Ichiro; Takano, Mikio; Kunoh, Hitoshi; Takada, Jun.

In: ACS Sustainable Chemistry and Engineering, Vol. 6, No. 1, 02.01.2018, p. 364-373.

Research output: Contribution to journalArticle

Kunoh, T, Takeda, M, Matsumoto, S, Suzuki, I, Takano, M, Kunoh, H & Takada, J 2018, 'Green Synthesis of Gold Nanoparticles Coupled with Nucleic Acid Oxidation', ACS Sustainable Chemistry and Engineering, vol. 6, no. 1, pp. 364-373. https://doi.org/10.1021/acssuschemeng.7b02610
Kunoh, Tatsuki ; Takeda, Minoru ; Matsumoto, Syuji ; Suzuki, Ichiro ; Takano, Mikio ; Kunoh, Hitoshi ; Takada, Jun. / Green Synthesis of Gold Nanoparticles Coupled with Nucleic Acid Oxidation. In: ACS Sustainable Chemistry and Engineering. 2018 ; Vol. 6, No. 1. pp. 364-373.
@article{b9ed5cf27b6841fd8e0ca40f2765d5a4,
title = "Green Synthesis of Gold Nanoparticles Coupled with Nucleic Acid Oxidation",
abstract = "Green synthesis of metal nanoparticles, especially gold nanoparticles (AuNPs), has attracted the great interest of scientists and engineers in the medical and pharmaceutical fields; thus, a variety of ecofriendly, energy- and cost-saving techniques have been developed. In this study, we found that cells of Leptothrix (iron-oxidizing bacteria) released extracellular RNA some of which could exist as a constituent of the cell-enclosing sheaths. As a part of studies of metal encrustation in the sheaths, here we show that RNA prepared from the Leptothrix cells can reduce Au(III) and spherical AuNPs eventually form when an aqueous HAuCl4 solution is added under ambient conditions. RNA and DNA of other organismal origins have the same ability. Of the nucleosides and nucleobases, only guanosine and guanine can form AuNPs. The DNA moiety, 2′-deoxyguanosine (dG) (used as a reference material), forms AuNPs when mixed with HAuCl4 solution, but 8-hydroxy-2′-deoxyguanosine (8-OHdG) does not, indicating that AuNP formation evidently depends on the reduction potential of the guanine moiety, not the sugar moiety. This finding is the first demonstration that spherical AuNPs of ca. 5 nm diameter can be obtained by simply adding guanine to HAuCl4 solution at ambient temperature; no other chemicals or physical treatments are needed.",
keywords = "8-OHdG, Green synthesis of AuNPs, Guanine, Nucleic acid oxidation, Nucleic acids, RNA in Leptothrix sheaths",
author = "Tatsuki Kunoh and Minoru Takeda and Syuji Matsumoto and Ichiro Suzuki and Mikio Takano and Hitoshi Kunoh and Jun Takada",
year = "2018",
month = "1",
day = "2",
doi = "10.1021/acssuschemeng.7b02610",
language = "English",
volume = "6",
pages = "364--373",
journal = "ACS Sustainable Chemistry and Engineering",
issn = "2168-0485",
publisher = "American Chemical Society",
number = "1",

}

TY - JOUR

T1 - Green Synthesis of Gold Nanoparticles Coupled with Nucleic Acid Oxidation

AU - Kunoh, Tatsuki

AU - Takeda, Minoru

AU - Matsumoto, Syuji

AU - Suzuki, Ichiro

AU - Takano, Mikio

AU - Kunoh, Hitoshi

AU - Takada, Jun

PY - 2018/1/2

Y1 - 2018/1/2

N2 - Green synthesis of metal nanoparticles, especially gold nanoparticles (AuNPs), has attracted the great interest of scientists and engineers in the medical and pharmaceutical fields; thus, a variety of ecofriendly, energy- and cost-saving techniques have been developed. In this study, we found that cells of Leptothrix (iron-oxidizing bacteria) released extracellular RNA some of which could exist as a constituent of the cell-enclosing sheaths. As a part of studies of metal encrustation in the sheaths, here we show that RNA prepared from the Leptothrix cells can reduce Au(III) and spherical AuNPs eventually form when an aqueous HAuCl4 solution is added under ambient conditions. RNA and DNA of other organismal origins have the same ability. Of the nucleosides and nucleobases, only guanosine and guanine can form AuNPs. The DNA moiety, 2′-deoxyguanosine (dG) (used as a reference material), forms AuNPs when mixed with HAuCl4 solution, but 8-hydroxy-2′-deoxyguanosine (8-OHdG) does not, indicating that AuNP formation evidently depends on the reduction potential of the guanine moiety, not the sugar moiety. This finding is the first demonstration that spherical AuNPs of ca. 5 nm diameter can be obtained by simply adding guanine to HAuCl4 solution at ambient temperature; no other chemicals or physical treatments are needed.

AB - Green synthesis of metal nanoparticles, especially gold nanoparticles (AuNPs), has attracted the great interest of scientists and engineers in the medical and pharmaceutical fields; thus, a variety of ecofriendly, energy- and cost-saving techniques have been developed. In this study, we found that cells of Leptothrix (iron-oxidizing bacteria) released extracellular RNA some of which could exist as a constituent of the cell-enclosing sheaths. As a part of studies of metal encrustation in the sheaths, here we show that RNA prepared from the Leptothrix cells can reduce Au(III) and spherical AuNPs eventually form when an aqueous HAuCl4 solution is added under ambient conditions. RNA and DNA of other organismal origins have the same ability. Of the nucleosides and nucleobases, only guanosine and guanine can form AuNPs. The DNA moiety, 2′-deoxyguanosine (dG) (used as a reference material), forms AuNPs when mixed with HAuCl4 solution, but 8-hydroxy-2′-deoxyguanosine (8-OHdG) does not, indicating that AuNP formation evidently depends on the reduction potential of the guanine moiety, not the sugar moiety. This finding is the first demonstration that spherical AuNPs of ca. 5 nm diameter can be obtained by simply adding guanine to HAuCl4 solution at ambient temperature; no other chemicals or physical treatments are needed.

KW - 8-OHdG

KW - Green synthesis of AuNPs

KW - Guanine

KW - Nucleic acid oxidation

KW - Nucleic acids

KW - RNA in Leptothrix sheaths

UR - http://www.scopus.com/inward/record.url?scp=85040044270&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85040044270&partnerID=8YFLogxK

U2 - 10.1021/acssuschemeng.7b02610

DO - 10.1021/acssuschemeng.7b02610

M3 - Article

AN - SCOPUS:85040044270

VL - 6

SP - 364

EP - 373

JO - ACS Sustainable Chemistry and Engineering

JF - ACS Sustainable Chemistry and Engineering

SN - 2168-0485

IS - 1

ER -