Grafting of encapsulated genetically modified cells secreting GDNF into the striatum of parkinsonian model rats

I. Date; T. Shingo; H. Yoshida; K. Fujiwara; K. Kobayashi; A. Takeuchi; T. Ohmoto

doi:10.3727/000000001783986486

Grafting of encapsulated genetically modified cells secreting GDNF into the striatum of parkinsonian model rats

I. Date, T. Shingo, H. Yoshida, K. Fujiwara, K. Kobayashi, A. Takeuchi, T. Ohmoto

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

In order to deliver glial cell line-derived neurotrophic factor (GDNF) into the brain, we have established a cell line that produces GDNF in a continuous fashion by genetic engineering. These cells were encapsulated and grafted into parkinsonian model rats that had received unilateral intrastriatal injection of 6-hydroxydopamine 2 weeks earlier. Neurochemical analysis showed that GDNF has been produced from the capsule for 6 months after grafting and histological analysis revealed good survival of GDNF-producing cells in the capsule 6 months after grafting. The density of nigrostriatal dopaminergic fibers in the striatum as well as the number of dopaminergic cell bodies in the substantia nigra recovered significantly after GDNF-producing cell grafting. These results suggest the possible application of GDNF-producing cell grafting for the treatment of Parkinson's disease.

Original language	English
Pages (from-to)	397-401
Number of pages	5
Journal	Cell Transplantation
Volume	10
Issue number	4-5
DOIs	https://doi.org/10.3727/000000001783986486
Publication status	Published - 2001

Keywords

Encapsulation
Glial cell line-derived neurotrophic factor GDNF)
Neural transplantation
Parkinson's disease

ASJC Scopus subject areas

Biomedical Engineering
Cell Biology
Transplantation

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10.3727/000000001783986486

Cite this

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abstract = "In order to deliver glial cell line-derived neurotrophic factor (GDNF) into the brain, we have established a cell line that produces GDNF in a continuous fashion by genetic engineering. These cells were encapsulated and grafted into parkinsonian model rats that had received unilateral intrastriatal injection of 6-hydroxydopamine 2 weeks earlier. Neurochemical analysis showed that GDNF has been produced from the capsule for 6 months after grafting and histological analysis revealed good survival of GDNF-producing cells in the capsule 6 months after grafting. The density of nigrostriatal dopaminergic fibers in the striatum as well as the number of dopaminergic cell bodies in the substantia nigra recovered significantly after GDNF-producing cell grafting. These results suggest the possible application of GDNF-producing cell grafting for the treatment of Parkinson's disease.",

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AU - Date, I.

AU - Shingo, T.

AU - Yoshida, H.

AU - Fujiwara, K.

AU - Kobayashi, K.

AU - Takeuchi, A.

AU - Ohmoto, T.

PY - 2001

Y1 - 2001

N2 - In order to deliver glial cell line-derived neurotrophic factor (GDNF) into the brain, we have established a cell line that produces GDNF in a continuous fashion by genetic engineering. These cells were encapsulated and grafted into parkinsonian model rats that had received unilateral intrastriatal injection of 6-hydroxydopamine 2 weeks earlier. Neurochemical analysis showed that GDNF has been produced from the capsule for 6 months after grafting and histological analysis revealed good survival of GDNF-producing cells in the capsule 6 months after grafting. The density of nigrostriatal dopaminergic fibers in the striatum as well as the number of dopaminergic cell bodies in the substantia nigra recovered significantly after GDNF-producing cell grafting. These results suggest the possible application of GDNF-producing cell grafting for the treatment of Parkinson's disease.

AB - In order to deliver glial cell line-derived neurotrophic factor (GDNF) into the brain, we have established a cell line that produces GDNF in a continuous fashion by genetic engineering. These cells were encapsulated and grafted into parkinsonian model rats that had received unilateral intrastriatal injection of 6-hydroxydopamine 2 weeks earlier. Neurochemical analysis showed that GDNF has been produced from the capsule for 6 months after grafting and histological analysis revealed good survival of GDNF-producing cells in the capsule 6 months after grafting. The density of nigrostriatal dopaminergic fibers in the striatum as well as the number of dopaminergic cell bodies in the substantia nigra recovered significantly after GDNF-producing cell grafting. These results suggest the possible application of GDNF-producing cell grafting for the treatment of Parkinson's disease.

KW - Encapsulation

KW - Glial cell line-derived neurotrophic factor GDNF)

KW - Neural transplantation

KW - Parkinson's disease

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Grafting of encapsulated genetically modified cells secreting GDNF into the striatum of parkinsonian model rats

Abstract

Keywords

ASJC Scopus subject areas

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