GPR155: Gene organization, multiple mRNA splice variants and expression in mouse central nervous system

Stefan Trifonov, Takeshi Houtani, Jun Ichi Shimizu, Satoko Hamada, Masahiko Kase, Masato Maruyama, Tetsuo Sugimoto

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Emerging evidence suggests that GPR155, an integral membrane protein related to G-protein coupled receptors, has specific roles in Huntington disease and autism spectrum disorders. This study reports the structural organization of mouse GPR155 gene and the generation of five variants (Variants 1-5) of GPR155 mRNA, including so far unknown four variants. Further, it presents the level of expression of GPR155 mRNA in different mouse tissues. The mRNAs for GPR155 are widely expressed in adult mouse tissues and during development. In situ hybridization was used to determine the distribution of GPR155 in mouse brain. The GPR155 mRNAs are widely distributed in forebrain regions and have more restricted distribution in the midbrain and hindbrain regions. The highest level of expression was in the lateral part of striatum and hippocampus. The expression pattern of GPR155 mRNAs in mouse striatum was very similar to that of cannabinoid receptor type 1. The predicted protein secondary structure indicated that GPR155 is a 17-TM protein, and Variant 1 and Variant 5 proteins have an intracellular, conserved DEP domain near the C-terminal.

Original languageEnglish
Pages (from-to)19-25
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume398
Issue number1
DOIs
Publication statusPublished - Jul 2010
Externally publishedYes

Keywords

  • CB1
  • GPR155
  • Gene organization
  • In situ hybridization
  • Striatum

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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