GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus

Yusuke Maeda, Toru Ide, Masato Koike, Yasuo Uchiyama, Taroh Kinoshita

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

The organelles within secretory and endocytotic pathways in mammalian cells have acidified lumens, and regulation of their acidic pH is critical for the trafficking, processing and glycosylation of cargo proteins and lipids, as well as the morphological integrity of the organelles. How organelle lumen acidification is regulated, and how luminal pH elevation disturbs these fundamental cellular processes, is largely unknown. Here, we describe a novel molecule involved in Golgi acidification. First, mutant cells defective in Golgi acidification were established that exhibited delayed protein transport, impaired glycosylation and Golgi disorganization. Using expression cloning, a novel Golgi-resident multi-transmembrane protein, named Golgi pH regulator (GPHR), was identified as being responsible for the mutant cells. After reconstitution in planar lipid bilayers, GPHR exhibited a voltage-dependent anion-channel activity that may function in counterion conductance. Thus, GPHR modulates Golgi functions through regulation of acidification.

Original languageEnglish
Pages (from-to)1135-1145
Number of pages11
JournalNature Cell Biology
Volume10
Issue number10
DOIs
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Golgi Apparatus
Anions
Organelles
Glycosylation
Voltage-Dependent Anion Channels
Secretory Pathway
Lipid Bilayers
Protein Transport
Organism Cloning
Lipids
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus. / Maeda, Yusuke; Ide, Toru; Koike, Masato; Uchiyama, Yasuo; Kinoshita, Taroh.

In: Nature Cell Biology, Vol. 10, No. 10, 2008, p. 1135-1145.

Research output: Contribution to journalArticle

Maeda, Yusuke ; Ide, Toru ; Koike, Masato ; Uchiyama, Yasuo ; Kinoshita, Taroh. / GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus. In: Nature Cell Biology. 2008 ; Vol. 10, No. 10. pp. 1135-1145.
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