Abstract
Coiled-coil proteins of the golgin family have been implicated in intra-Golgi transport through tethering coat protein complex I (COPI) vesicles. The p115-golgin tether is the best studied, and here we characterize the golgin-84-CASP tether. The vesicles bound by this tether were strikingly different from those bound by the p115-golgin tether in that they lacked members of the p24 family of putative cargo receptors and contained enzymes instead of anterograde cargo. Microinjected golgin-84 or CASP also inhibited Golgi-enzyme transport to the endoplasmic reticulum, further implicating this tether in retrograde transport. These and other golgins may modulate the flow patterns within the Golgi stack.
| Original language | English |
|---|---|
| Pages (from-to) | 1095-1098 |
| Number of pages | 4 |
| Journal | Science |
| Volume | 307 |
| Issue number | 5712 |
| DOIs | |
| Publication status | Published - Feb 18 2005 |
| Externally published | Yes |
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Cite this
Golgin tethers define subpopulations of COPI vesicles. / Malsam, Jörg; Satoh, Ayano; Pelletier, Laurence; Warren, Graham.
In: Science, Vol. 307, No. 5712, 18.02.2005, p. 1095-1098.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Golgin tethers define subpopulations of COPI vesicles
AU - Malsam, Jörg
AU - Satoh, Ayano
AU - Pelletier, Laurence
AU - Warren, Graham
PY - 2005/2/18
Y1 - 2005/2/18
N2 - Coiled-coil proteins of the golgin family have been implicated in intra-Golgi transport through tethering coat protein complex I (COPI) vesicles. The p115-golgin tether is the best studied, and here we characterize the golgin-84-CASP tether. The vesicles bound by this tether were strikingly different from those bound by the p115-golgin tether in that they lacked members of the p24 family of putative cargo receptors and contained enzymes instead of anterograde cargo. Microinjected golgin-84 or CASP also inhibited Golgi-enzyme transport to the endoplasmic reticulum, further implicating this tether in retrograde transport. These and other golgins may modulate the flow patterns within the Golgi stack.
AB - Coiled-coil proteins of the golgin family have been implicated in intra-Golgi transport through tethering coat protein complex I (COPI) vesicles. The p115-golgin tether is the best studied, and here we characterize the golgin-84-CASP tether. The vesicles bound by this tether were strikingly different from those bound by the p115-golgin tether in that they lacked members of the p24 family of putative cargo receptors and contained enzymes instead of anterograde cargo. Microinjected golgin-84 or CASP also inhibited Golgi-enzyme transport to the endoplasmic reticulum, further implicating this tether in retrograde transport. These and other golgins may modulate the flow patterns within the Golgi stack.
UR - http://www.scopus.com/inward/record.url?scp=13844317835&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=13844317835&partnerID=8YFLogxK
U2 - 10.1126/science.1108061
DO - 10.1126/science.1108061
M3 - Article
C2 - 15718469
AN - SCOPUS:13844317835
VL - 307
SP - 1095
EP - 1098
JO - Science
JF - Science
SN - 0036-8075
IS - 5712
ER -