Golgin-84 is a rab1 binding partner involved in Golgi structure

Ayaho Satoh, Yanzhuang Wang, Jörg Malsam, Matthew B. Beard, Graham Warren

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Members of the golgin family of coiled-coil proteins have been implicated in the tethering of vesicles to Golgi membranes and cisternal membranes to each other. Many also bind to rab GTPases. Golgin-84 is a membrane-anchored golgin that we now show binds preferentially to the GTP form of the rab1 GTPase. It is also present throughout the Golgi stack by immuno-EM. Antibodies to golgin-84 inhibit stacking of cisternal membranes in a cell-free assay for Golgi reassembly, whereas the cytoplasmic domain of golgin-84 stimulates stacking and increases the length of re-assembled stacks. Transient expression of golgin-84 in NRK cells helps prevent the disassembly of the Golgi apparatus normally triggered by treatment with brefeldin A. Together these data suggest that golgin-84 is involved in generating and maintaining the architecture of the Golgi apparatus.

Original languageEnglish
Pages (from-to)153-161
Number of pages9
JournalTraffic
Volume4
Issue number3
DOIs
Publication statusPublished - Mar 1 2003
Externally publishedYes

Keywords

  • Cisterna
  • GTPase
  • Golgi
  • Golgin
  • Mitosis
  • Rab

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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