Abstract
Members of the golgin family of coiled-coil proteins have been implicated in the tethering of vesicles to Golgi membranes and cisternal membranes to each other. Many also bind to rab GTPases. Golgin-84 is a membrane-anchored golgin that we now show binds preferentially to the GTP form of the rab1 GTPase. It is also present throughout the Golgi stack by immuno-EM. Antibodies to golgin-84 inhibit stacking of cisternal membranes in a cell-free assay for Golgi reassembly, whereas the cytoplasmic domain of golgin-84 stimulates stacking and increases the length of re-assembled stacks. Transient expression of golgin-84 in NRK cells helps prevent the disassembly of the Golgi apparatus normally triggered by treatment with brefeldin A. Together these data suggest that golgin-84 is involved in generating and maintaining the architecture of the Golgi apparatus.
Original language | English |
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Pages (from-to) | 153-161 |
Number of pages | 9 |
Journal | Traffic |
Volume | 4 |
Issue number | 3 |
DOIs | |
Publication status | Published - Mar 1 2003 |
Externally published | Yes |
Keywords
- Cisterna
- GTPase
- Golgi
- Golgin
- Mitosis
- Rab
ASJC Scopus subject areas
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology