Golgin-84 is a rab1 binding partner involved in Golgi structure

Ayaho Satoh; Yanzhuang Wang; Jörg Malsam; Matthew B. Beard; Graham Warren

doi:10.1034/j.1600-0854.2003.00103.x

Golgin-84 is a rab1 binding partner involved in Golgi structure

Ayaho Satoh, Yanzhuang Wang, Jörg Malsam, Matthew B. Beard, Graham Warren

Research output: Contribution to journal › Article › peer-review

97 Citations (Scopus)

Abstract

Members of the golgin family of coiled-coil proteins have been implicated in the tethering of vesicles to Golgi membranes and cisternal membranes to each other. Many also bind to rab GTPases. Golgin-84 is a membrane-anchored golgin that we now show binds preferentially to the GTP form of the rab1 GTPase. It is also present throughout the Golgi stack by immuno-EM. Antibodies to golgin-84 inhibit stacking of cisternal membranes in a cell-free assay for Golgi reassembly, whereas the cytoplasmic domain of golgin-84 stimulates stacking and increases the length of re-assembled stacks. Transient expression of golgin-84 in NRK cells helps prevent the disassembly of the Golgi apparatus normally triggered by treatment with brefeldin A. Together these data suggest that golgin-84 is involved in generating and maintaining the architecture of the Golgi apparatus.

Original language	English
Pages (from-to)	153-161
Number of pages	9
Journal	Traffic
Volume	4
Issue number	3
DOIs	https://doi.org/10.1034/j.1600-0854.2003.00103.x
Publication status	Published - Mar 1 2003
Externally published	Yes

Keywords

Cisterna
GTPase
Golgi
Golgin
Mitosis
Rab

ASJC Scopus subject areas

Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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abstract = "Members of the golgin family of coiled-coil proteins have been implicated in the tethering of vesicles to Golgi membranes and cisternal membranes to each other. Many also bind to rab GTPases. Golgin-84 is a membrane-anchored golgin that we now show binds preferentially to the GTP form of the rab1 GTPase. It is also present throughout the Golgi stack by immuno-EM. Antibodies to golgin-84 inhibit stacking of cisternal membranes in a cell-free assay for Golgi reassembly, whereas the cytoplasmic domain of golgin-84 stimulates stacking and increases the length of re-assembled stacks. Transient expression of golgin-84 in NRK cells helps prevent the disassembly of the Golgi apparatus normally triggered by treatment with brefeldin A. Together these data suggest that golgin-84 is involved in generating and maintaining the architecture of the Golgi apparatus.",

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AU - Satoh, Ayaho

AU - Wang, Yanzhuang

AU - Malsam, Jörg

AU - Beard, Matthew B.

AU - Warren, Graham

PY - 2003/3/1

Y1 - 2003/3/1

N2 - Members of the golgin family of coiled-coil proteins have been implicated in the tethering of vesicles to Golgi membranes and cisternal membranes to each other. Many also bind to rab GTPases. Golgin-84 is a membrane-anchored golgin that we now show binds preferentially to the GTP form of the rab1 GTPase. It is also present throughout the Golgi stack by immuno-EM. Antibodies to golgin-84 inhibit stacking of cisternal membranes in a cell-free assay for Golgi reassembly, whereas the cytoplasmic domain of golgin-84 stimulates stacking and increases the length of re-assembled stacks. Transient expression of golgin-84 in NRK cells helps prevent the disassembly of the Golgi apparatus normally triggered by treatment with brefeldin A. Together these data suggest that golgin-84 is involved in generating and maintaining the architecture of the Golgi apparatus.

AB - Members of the golgin family of coiled-coil proteins have been implicated in the tethering of vesicles to Golgi membranes and cisternal membranes to each other. Many also bind to rab GTPases. Golgin-84 is a membrane-anchored golgin that we now show binds preferentially to the GTP form of the rab1 GTPase. It is also present throughout the Golgi stack by immuno-EM. Antibodies to golgin-84 inhibit stacking of cisternal membranes in a cell-free assay for Golgi reassembly, whereas the cytoplasmic domain of golgin-84 stimulates stacking and increases the length of re-assembled stacks. Transient expression of golgin-84 in NRK cells helps prevent the disassembly of the Golgi apparatus normally triggered by treatment with brefeldin A. Together these data suggest that golgin-84 is involved in generating and maintaining the architecture of the Golgi apparatus.

KW - Cisterna

KW - GTPase

KW - Golgi

KW - Golgin

KW - Mitosis

KW - Rab

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JO - Traffic

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ER -

Golgin-84 is a rab1 binding partner involved in Golgi structure

Abstract

Keywords

ASJC Scopus subject areas

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