GOLDEN 2-LIKE transcription factors for chloroplast development affect ozone tolerance through the regulation of stomatal movement

Yukari Nagatoshi, Nobutaka Mitsuda, Maki Hayashi, Shin Ichiro Inoue, Eiji Okuma, Akihiro Kubo, Yoshiyuki Murata, Mitsunori Seo, Hikaru Saji, Toshinori Kinoshita, Masaru Ohme-Takagi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Stomatal movements regulate gas exchange, thus directly affecting the efficiency of photosynthesis and the sensitivity of plants to air pollutants such as ozone. The GARP family transcription factors GOLDEN 2-LIKE1 (GLK1) and GLK2 have known functions in chloroplast development. Here, we show that Arabidopsis thaliana (A. thaliana) plants expressing the chimeric repressors for GLK1 and-2 (GLK1/2-SRDX) exhibited a closed-stomata phenotype and strong tolerance to ozone. By contrast, plants that overexpress GLK1/2 exhibited an open-stomata phenotype and higher sensitivity to ozone. The plants expressing GLK1-SRDX had reduced expression of the genes for inwardly rectifying K+ (K+ in) channels and reduced K+ in channel activity. Abscisic acid treatment did not affect the stomatal phenotype of 35S:GLK1/2-SRDX plants or the transcriptional activity for K+ in channel gene, indicating that GLK1/2 act independently of abscisic acid signaling. Our results indicate that GLK1/2 positively regulate the expression of genes for K+ in channels and promote stomatal opening. Because the chimeric GLK1-SRDX repressor driven by a guard cell-specific promoter induced a closed-stomata phenotype without affecting chloroplast development in mesophyll cells, modulating GLK1/2 activity may provide an effective tool to control stomatal movements and thus to confer resistance to air pollutants.

Original languageEnglish
Pages (from-to)4218-4223
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number15
DOIs
Publication statusPublished - Apr 12 2016

Fingerprint

Ozone
Chloroplasts
Transcription Factors
Phenotype
Air Pollutants
Abscisic Acid
Mesophyll Cells
Gene Expression
Photosynthesis
Arabidopsis
Gases
Genes

Keywords

  • K channel
  • Ozone
  • Repressor
  • Stomatal movement
  • Transcription factor

ASJC Scopus subject areas

  • General

Cite this

GOLDEN 2-LIKE transcription factors for chloroplast development affect ozone tolerance through the regulation of stomatal movement. / Nagatoshi, Yukari; Mitsuda, Nobutaka; Hayashi, Maki; Inoue, Shin Ichiro; Okuma, Eiji; Kubo, Akihiro; Murata, Yoshiyuki; Seo, Mitsunori; Saji, Hikaru; Kinoshita, Toshinori; Ohme-Takagi, Masaru.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 15, 12.04.2016, p. 4218-4223.

Research output: Contribution to journalArticle

Nagatoshi, Yukari ; Mitsuda, Nobutaka ; Hayashi, Maki ; Inoue, Shin Ichiro ; Okuma, Eiji ; Kubo, Akihiro ; Murata, Yoshiyuki ; Seo, Mitsunori ; Saji, Hikaru ; Kinoshita, Toshinori ; Ohme-Takagi, Masaru. / GOLDEN 2-LIKE transcription factors for chloroplast development affect ozone tolerance through the regulation of stomatal movement. In: Proceedings of the National Academy of Sciences of the United States of America. 2016 ; Vol. 113, No. 15. pp. 4218-4223.
@article{1dfeaa559d9b46fb8400968931127652,
title = "GOLDEN 2-LIKE transcription factors for chloroplast development affect ozone tolerance through the regulation of stomatal movement",
abstract = "Stomatal movements regulate gas exchange, thus directly affecting the efficiency of photosynthesis and the sensitivity of plants to air pollutants such as ozone. The GARP family transcription factors GOLDEN 2-LIKE1 (GLK1) and GLK2 have known functions in chloroplast development. Here, we show that Arabidopsis thaliana (A. thaliana) plants expressing the chimeric repressors for GLK1 and-2 (GLK1/2-SRDX) exhibited a closed-stomata phenotype and strong tolerance to ozone. By contrast, plants that overexpress GLK1/2 exhibited an open-stomata phenotype and higher sensitivity to ozone. The plants expressing GLK1-SRDX had reduced expression of the genes for inwardly rectifying K+ (K+ in) channels and reduced K+ in channel activity. Abscisic acid treatment did not affect the stomatal phenotype of 35S:GLK1/2-SRDX plants or the transcriptional activity for K+ in channel gene, indicating that GLK1/2 act independently of abscisic acid signaling. Our results indicate that GLK1/2 positively regulate the expression of genes for K+ in channels and promote stomatal opening. Because the chimeric GLK1-SRDX repressor driven by a guard cell-specific promoter induced a closed-stomata phenotype without affecting chloroplast development in mesophyll cells, modulating GLK1/2 activity may provide an effective tool to control stomatal movements and thus to confer resistance to air pollutants.",
keywords = "K channel, Ozone, Repressor, Stomatal movement, Transcription factor",
author = "Yukari Nagatoshi and Nobutaka Mitsuda and Maki Hayashi and Inoue, {Shin Ichiro} and Eiji Okuma and Akihiro Kubo and Yoshiyuki Murata and Mitsunori Seo and Hikaru Saji and Toshinori Kinoshita and Masaru Ohme-Takagi",
year = "2016",
month = "4",
day = "12",
doi = "10.1073/pnas.1513093113",
language = "English",
volume = "113",
pages = "4218--4223",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "15",

}

TY - JOUR

T1 - GOLDEN 2-LIKE transcription factors for chloroplast development affect ozone tolerance through the regulation of stomatal movement

AU - Nagatoshi, Yukari

AU - Mitsuda, Nobutaka

AU - Hayashi, Maki

AU - Inoue, Shin Ichiro

AU - Okuma, Eiji

AU - Kubo, Akihiro

AU - Murata, Yoshiyuki

AU - Seo, Mitsunori

AU - Saji, Hikaru

AU - Kinoshita, Toshinori

AU - Ohme-Takagi, Masaru

PY - 2016/4/12

Y1 - 2016/4/12

N2 - Stomatal movements regulate gas exchange, thus directly affecting the efficiency of photosynthesis and the sensitivity of plants to air pollutants such as ozone. The GARP family transcription factors GOLDEN 2-LIKE1 (GLK1) and GLK2 have known functions in chloroplast development. Here, we show that Arabidopsis thaliana (A. thaliana) plants expressing the chimeric repressors for GLK1 and-2 (GLK1/2-SRDX) exhibited a closed-stomata phenotype and strong tolerance to ozone. By contrast, plants that overexpress GLK1/2 exhibited an open-stomata phenotype and higher sensitivity to ozone. The plants expressing GLK1-SRDX had reduced expression of the genes for inwardly rectifying K+ (K+ in) channels and reduced K+ in channel activity. Abscisic acid treatment did not affect the stomatal phenotype of 35S:GLK1/2-SRDX plants or the transcriptional activity for K+ in channel gene, indicating that GLK1/2 act independently of abscisic acid signaling. Our results indicate that GLK1/2 positively regulate the expression of genes for K+ in channels and promote stomatal opening. Because the chimeric GLK1-SRDX repressor driven by a guard cell-specific promoter induced a closed-stomata phenotype without affecting chloroplast development in mesophyll cells, modulating GLK1/2 activity may provide an effective tool to control stomatal movements and thus to confer resistance to air pollutants.

AB - Stomatal movements regulate gas exchange, thus directly affecting the efficiency of photosynthesis and the sensitivity of plants to air pollutants such as ozone. The GARP family transcription factors GOLDEN 2-LIKE1 (GLK1) and GLK2 have known functions in chloroplast development. Here, we show that Arabidopsis thaliana (A. thaliana) plants expressing the chimeric repressors for GLK1 and-2 (GLK1/2-SRDX) exhibited a closed-stomata phenotype and strong tolerance to ozone. By contrast, plants that overexpress GLK1/2 exhibited an open-stomata phenotype and higher sensitivity to ozone. The plants expressing GLK1-SRDX had reduced expression of the genes for inwardly rectifying K+ (K+ in) channels and reduced K+ in channel activity. Abscisic acid treatment did not affect the stomatal phenotype of 35S:GLK1/2-SRDX plants or the transcriptional activity for K+ in channel gene, indicating that GLK1/2 act independently of abscisic acid signaling. Our results indicate that GLK1/2 positively regulate the expression of genes for K+ in channels and promote stomatal opening. Because the chimeric GLK1-SRDX repressor driven by a guard cell-specific promoter induced a closed-stomata phenotype without affecting chloroplast development in mesophyll cells, modulating GLK1/2 activity may provide an effective tool to control stomatal movements and thus to confer resistance to air pollutants.

KW - K channel

KW - Ozone

KW - Repressor

KW - Stomatal movement

KW - Transcription factor

UR - http://www.scopus.com/inward/record.url?scp=84963782515&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84963782515&partnerID=8YFLogxK

U2 - 10.1073/pnas.1513093113

DO - 10.1073/pnas.1513093113

M3 - Article

VL - 113

SP - 4218

EP - 4223

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 15

ER -