Gnom-like1/ermo1 and sec24a/ermo2 are required for maintenance of endoplasmic reticulum morphology inarabidopsis thaliana

Ryohei Thomas Nakano, Ryo Matsushima, Haruko Ueda, Kentaro Tamura, Tomoo Shimada, Lixin Li, Yasuko Hayashi, Maki Kondo, Mikio Nishimura, Ikuko Hara-Nishimura

Research output: Contribution to journalArticle

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Abstract

The endoplasmic reticulum (ER) is composed of tubules, sheets, and three-way junctions, resulting in a highly conserved polygonal network in all eukaryotes. The molecular mechanisms responsible for the organization of these structures are obscure. To identify novel factors responsible for ER morphology, we employed a forward genetic approach using a transgenic Arabidopsis thaliana plant (GFP-h) with fluorescently labeled ER. We isolated two mutants with defects in ER morphology and designated them endoplasmic reticulum morphology1 (ermo1) and ermo2. The cells of both mutants developed a number of ER-derived spherical bodies, ;1 mm in diameter, in addition to the typical polygonal network of ER. The spherical bodies were distributed throughout the ermo1 cells, while they formed a large aggregate in ermo2 cells. We identified the responsible gene for ermo1 to be GNOM-LIKE1 (GNL1) and the gene for ermo2 to be SEC24a. Homologs of both GNL1 and SEC24a are involved in membrane trafficking between the ER and Golgi in yeast and animal cells. Our findings, however, suggest that GNL1/ERMO1 and SEC24a/ERMO2 have a novel function in ER morphology in higher plants.

Original languageEnglish
Pages (from-to)3672-3685
Number of pages14
JournalPlant Cell
Volume21
Issue number11
DOIs
Publication statusPublished - Nov 2009

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Endoplasmic Reticulum
endoplasmic reticulum
Maintenance
mutants
cells
cell aggregates
Eukaryota
Arabidopsis
Genes
eukaryotic cells
genes
Arabidopsis thaliana
Yeasts
genetically modified organisms
yeasts
Membranes

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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Gnom-like1/ermo1 and sec24a/ermo2 are required for maintenance of endoplasmic reticulum morphology inarabidopsis thaliana. / Nakano, Ryohei Thomas; Matsushima, Ryo; Ueda, Haruko; Tamura, Kentaro; Shimada, Tomoo; Li, Lixin; Hayashi, Yasuko; Kondo, Maki; Nishimura, Mikio; Hara-Nishimura, Ikuko.

In: Plant Cell, Vol. 21, No. 11, 11.2009, p. 3672-3685.

Research output: Contribution to journalArticle

Nakano, RT, Matsushima, R, Ueda, H, Tamura, K, Shimada, T, Li, L, Hayashi, Y, Kondo, M, Nishimura, M & Hara-Nishimura, I 2009, 'Gnom-like1/ermo1 and sec24a/ermo2 are required for maintenance of endoplasmic reticulum morphology inarabidopsis thaliana', Plant Cell, vol. 21, no. 11, pp. 3672-3685. https://doi.org/10.1105/tpc.109.068270
Nakano, Ryohei Thomas ; Matsushima, Ryo ; Ueda, Haruko ; Tamura, Kentaro ; Shimada, Tomoo ; Li, Lixin ; Hayashi, Yasuko ; Kondo, Maki ; Nishimura, Mikio ; Hara-Nishimura, Ikuko. / Gnom-like1/ermo1 and sec24a/ermo2 are required for maintenance of endoplasmic reticulum morphology inarabidopsis thaliana. In: Plant Cell. 2009 ; Vol. 21, No. 11. pp. 3672-3685.
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