Glycosylation of flagellin from Pseudomonas syringae pv. tabaci 6605 contributes to evasion of host tobacco plant surveillance system

Fumiko Taguchi, Tomoko Suzuki, Kasumi Takeuchi, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Pseudomonas syringae pv. tabaci (Pta) possesses a genetic region composed of two open reading frames (ORFs), fgt1 and fgt2, that are involved in glycosylation of flagellin. The deletion mutant Δfgt1 produced non-glycosylated flagellin, and exhibited reduced ability to cause disease in the host tobacco plant. Flagellin is known to induce plant defense responses, and the recognition of flagellin by Arabidopsis thaliana is mediated by a conserved N-terminal region, flg22, in flagellin and a leucine-rich repeat domain in the FLS2 receptor. Because flg22 localizes inside the flagellum, polymerized flagellum needs to be dissociated to be recognized. Therefore, the effect of glycosylation on flagella stability was investigated. The polymerized flagella from glycosylated flagellins were more resistant to heat treatment than those from non-glycosylated flagellins, suggesting that the glycosylation of flagellin contributes to the structural stability of flagella and prevents exposure of the flg22 region. Polymerized flagella from Pta Δfgt1 flagellin and depolymerized and glycosylated flagellin from Pta wild type induced cell death and callose deposition, and inhibited seedling growth in tobacco more effectively, whereas polymerized flagella from Pta wild-type flagellin caused a low level of these responses. These results suggest Pta might have evolved the flagellin glycosylation system to evade detection and defense response of a host by increasing flagella stability and suppressing their dissociation.

Original languageEnglish
Pages (from-to)11-17
Number of pages7
JournalPhysiological and Molecular Plant Pathology
Volume74
Issue number1
DOIs
Publication statusPublished - Jan 2009

Fingerprint

Pseudomonas syringae pv. tabaci
Pseudomonas syringae
Flagellin
flagellin
glycosylation
Glycosylation
Tobacco
tobacco
Flagella
flagellum
monitoring
callose
Seedlings
Arabidopsis
Leucine
Open Reading Frames
seedling growth
leucine

Keywords

  • Defense response
  • Flagellar stability
  • Flagellin
  • Glycosylation
  • Host specificity
  • MAMP

ASJC Scopus subject areas

  • Plant Science
  • Genetics

Cite this

Glycosylation of flagellin from Pseudomonas syringae pv. tabaci 6605 contributes to evasion of host tobacco plant surveillance system. / Taguchi, Fumiko; Suzuki, Tomoko; Takeuchi, Kasumi; Inagaki, Yoshishige; Toyoda, Kazuhiro; Shiraishi, Tomonori; Ichinose, Yuki.

In: Physiological and Molecular Plant Pathology, Vol. 74, No. 1, 01.2009, p. 11-17.

Research output: Contribution to journalArticle

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