Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptides

Pierre Buscaill, Balakumaran Chandrasekar, Nattapong Sanguankiattichai, Jiorgos Kourelis, Farnusch Kaschani, Emma L. Thomas, Kyoko Morimoto, Markus Kaiser, Gail M. Preston, Yuki Ichinose, Renier A.L. Van Der Hoorn

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Plants and animals recognize conserved flagellin fragments as a signature of bacterial invasion. These immunogenic elicitor peptides are embedded in the flagellin polymer and require hydrolytic release before they can activate cell surface receptors. Although much of flagellin signaling is understood, little is known about the release of immunogenic fragments. We discovered that plant-secreted b-galactosidase 1 (BGAL1) of Nicotiana benthamiana promotes hydrolytic elicitor release and acts in immunity against pathogenic Pseudomonas syringae strains only when they carry a terminal modified viosamine (mVio) in the flagellin O-glycan. In counter defense, P. syringae pathovars evade host immunity by using BGAL1-resistant O-glycans or by producing a BGAL1 inhibitor. Polymorphic glycans on flagella are common to plant and animal pathogenic bacteria and represent an important determinant of host immunity to bacterial pathogens.

Original languageEnglish
Article numbereaav0748
JournalScience
Volume364
Issue number6436
DOIs
Publication statusPublished - Apr 12 2019

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Flagellin
Glycoside Hydrolases
Galactosidases
Polysaccharides
Pseudomonas syringae
Immunity
Peptides
Flagella
Cell Surface Receptors
Tobacco
Polymers
Bacteria

ASJC Scopus subject areas

  • General

Cite this

Buscaill, P., Chandrasekar, B., Sanguankiattichai, N., Kourelis, J., Kaschani, F., Thomas, E. L., ... Van Der Hoorn, R. A. L. (2019). Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptides. Science, 364(6436), [eaav0748]. https://doi.org/10.1126/science.aav0748

Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptides. / Buscaill, Pierre; Chandrasekar, Balakumaran; Sanguankiattichai, Nattapong; Kourelis, Jiorgos; Kaschani, Farnusch; Thomas, Emma L.; Morimoto, Kyoko; Kaiser, Markus; Preston, Gail M.; Ichinose, Yuki; Van Der Hoorn, Renier A.L.

In: Science, Vol. 364, No. 6436, eaav0748, 12.04.2019.

Research output: Contribution to journalArticle

Buscaill, P, Chandrasekar, B, Sanguankiattichai, N, Kourelis, J, Kaschani, F, Thomas, EL, Morimoto, K, Kaiser, M, Preston, GM, Ichinose, Y & Van Der Hoorn, RAL 2019, 'Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptides', Science, vol. 364, no. 6436, eaav0748. https://doi.org/10.1126/science.aav0748
Buscaill P, Chandrasekar B, Sanguankiattichai N, Kourelis J, Kaschani F, Thomas EL et al. Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptides. Science. 2019 Apr 12;364(6436). eaav0748. https://doi.org/10.1126/science.aav0748
Buscaill, Pierre ; Chandrasekar, Balakumaran ; Sanguankiattichai, Nattapong ; Kourelis, Jiorgos ; Kaschani, Farnusch ; Thomas, Emma L. ; Morimoto, Kyoko ; Kaiser, Markus ; Preston, Gail M. ; Ichinose, Yuki ; Van Der Hoorn, Renier A.L. / Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptides. In: Science. 2019 ; Vol. 364, No. 6436.
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