Abstract
Although it has been found that plant endo-β-N-acetylglucosaminidase shows strong activity towards denatured glycoproteins and glycopeptides with high-mannose type N-glycans and free high-mannose type N-glycans bearing the chitobiosyl unit, the endogenous substrates for plant endoglycosidase have not yet been identified. Recently we purified and characterized an endo-β-N-acetylglucosaminidase from rice culture cells and identified the gene encoded (Maeda, M., and Kimura, Y., Trends Glycosci. Glycotech., 17, 205-214 (2005)). Furthermore, we found structural features of free N-glycans in the cells, indicating that high-mannose type species (Man 9-5GlcNAc1) occur at concentration of several micromolar (μM). Hence, in this study we analyzed glycoform of N-glycans linked to glycoproteins expressed in rice culture cells to see whether endogenous glycoproteinous substrate occurs in reasonable amounts. Structural analysis revealed that more than 95% of total N-glycans linked to glycoproteins in the rice cells had the plant complex type structure, including Lewis a epitope-harboring type, although high-mannose type structures account for less than 5% of total N-glycans.
| Original language | English |
|---|---|
| Pages (from-to) | 1356-1363 |
| Number of pages | 8 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 70 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2006 |
Fingerprint
Keywords
- N-glycan metabolism
- N-glycan structures
- Oriza sativa
- Rice glycoprotein
ASJC Scopus subject areas
- Bioengineering
- Biotechnology
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Chemistry (miscellaneous)
- Applied Microbiology and Biotechnology
- Food Science
Cite this
Glycoform analysis of N-glycans linked to glycoproteins expressed in rice culture cells : Predominant occurrence of complex type N-glycans. / Maeda, Megumi; Kimura, Yoshinobu.
In: Bioscience, Biotechnology and Biochemistry, Vol. 70, No. 6, 2006, p. 1356-1363.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Glycoform analysis of N-glycans linked to glycoproteins expressed in rice culture cells
T2 - Predominant occurrence of complex type N-glycans
AU - Maeda, Megumi
AU - Kimura, Yoshinobu
PY - 2006
Y1 - 2006
N2 - Although it has been found that plant endo-β-N-acetylglucosaminidase shows strong activity towards denatured glycoproteins and glycopeptides with high-mannose type N-glycans and free high-mannose type N-glycans bearing the chitobiosyl unit, the endogenous substrates for plant endoglycosidase have not yet been identified. Recently we purified and characterized an endo-β-N-acetylglucosaminidase from rice culture cells and identified the gene encoded (Maeda, M., and Kimura, Y., Trends Glycosci. Glycotech., 17, 205-214 (2005)). Furthermore, we found structural features of free N-glycans in the cells, indicating that high-mannose type species (Man 9-5GlcNAc1) occur at concentration of several micromolar (μM). Hence, in this study we analyzed glycoform of N-glycans linked to glycoproteins expressed in rice culture cells to see whether endogenous glycoproteinous substrate occurs in reasonable amounts. Structural analysis revealed that more than 95% of total N-glycans linked to glycoproteins in the rice cells had the plant complex type structure, including Lewis a epitope-harboring type, although high-mannose type structures account for less than 5% of total N-glycans.
AB - Although it has been found that plant endo-β-N-acetylglucosaminidase shows strong activity towards denatured glycoproteins and glycopeptides with high-mannose type N-glycans and free high-mannose type N-glycans bearing the chitobiosyl unit, the endogenous substrates for plant endoglycosidase have not yet been identified. Recently we purified and characterized an endo-β-N-acetylglucosaminidase from rice culture cells and identified the gene encoded (Maeda, M., and Kimura, Y., Trends Glycosci. Glycotech., 17, 205-214 (2005)). Furthermore, we found structural features of free N-glycans in the cells, indicating that high-mannose type species (Man 9-5GlcNAc1) occur at concentration of several micromolar (μM). Hence, in this study we analyzed glycoform of N-glycans linked to glycoproteins expressed in rice culture cells to see whether endogenous glycoproteinous substrate occurs in reasonable amounts. Structural analysis revealed that more than 95% of total N-glycans linked to glycoproteins in the rice cells had the plant complex type structure, including Lewis a epitope-harboring type, although high-mannose type structures account for less than 5% of total N-glycans.
KW - N-glycan metabolism
KW - N-glycan structures
KW - Oriza sativa
KW - Rice glycoprotein
UR - http://www.scopus.com/inward/record.url?scp=33745483793&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745483793&partnerID=8YFLogxK
U2 - 10.1271/bbb.50637
DO - 10.1271/bbb.50637
M3 - Article
C2 - 16794314
AN - SCOPUS:33745483793
VL - 70
SP - 1356
EP - 1363
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 6
ER -