Glycoform analysis of Japanese cypress pollen allergen, Cha o 1: A comparison of the glycoforms of cedar and Cypress pollen allergens

Yoshinobu Kimura, Misao Kuroki, Megumi Maeda, Mitsuhiro Okano, Minehiko Yokoyama, Kosuke Kino

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A Japanese cypress (Chamaecyparis obtusa) pollen allergen, Cha o 1, is one of the major allergens that cause allergic pollinosis in Japan. Although it has been found that Cha o 1 is glycosylated and that the amino acid sequence is highly homologous with that of Japanese cedar pollen allergen (Cry j 1), the structure of N-glycans linked to Cha o 1 remains to be determined. In this study, therefore, we analyzed the structures of the N-glycans of Cha o1. The N-glycans were liberated by hydrazinolysis from purified Cha o 1, and the resulting sugar chains were N-acetylated and pyridylaminated. The structures of pyridylaminated N-glycans were analyzed by a combination of exoglycosidase digestion, two dimensional (2D-) sugar chain mapping, and electrospray ionization mass spectrometry analysis. Structural analysis indicated that the major N-glycan structure of Cha o1 is GlcNAc2Man3Xyl1-Fuc1GlcNAc2 (89%), and that high-mannose type structures (Man9GlcNAc2, Man7GlcNAc2) occur as minor components (11%).

Original languageEnglish
Pages (from-to)485-491
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number2
DOIs
Publication statusPublished - 2008

Fingerprint

Cupressus
Allergens
Pollen
allergens
Polysaccharides
polysaccharides
pollen
Sugars
Electrospray ionization
Chamaecyparis
Structural analysis
Mass spectrometry
Amino acids
Cryptomeria
hay fever
sugars
Chamaecyparis obtusa
Cryptomeria japonica
Seasonal Allergic Rhinitis
Electrospray Ionization Mass Spectrometry

Keywords

  • Antigenic oligosaccharide
  • Cha o 1
  • Chamaecyparis obtusa
  • Japanese cypress pollen allergen
  • N-glycan structure

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Glycoform analysis of Japanese cypress pollen allergen, Cha o 1 : A comparison of the glycoforms of cedar and Cypress pollen allergens. / Kimura, Yoshinobu; Kuroki, Misao; Maeda, Megumi; Okano, Mitsuhiro; Yokoyama, Minehiko; Kino, Kosuke.

In: Bioscience, Biotechnology and Biochemistry, Vol. 72, No. 2, 2008, p. 485-491.

Research output: Contribution to journalArticle

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