Glycocalyx degradation in retinal and choroidal capillary endothelium in rats with diabetes and hypertension

Fumiaki Kumase, Yuki Morizane, Satoshi Mohri, Ippei Takasu, Aiji Ohtsuka, Hiroshi Ohtsukri

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Endothelial glycocalyx (GCX) has been reported as a protective factor for vascular endothelial cells (VEC) in diabetes and hypertension. However, the involvement of GCX impairment in ocular vasculopathy remains unclear. We evaluated the changes in the GCX thicknesses of the retinal and choroidal capillaries in rats with diabetes and hypertension by cationic colloidal iron staining using a transmission electron microscope. In the control group, the mean (standard error of the mean) thicknesses of retinal and choroidal GCX were 60.2 (1.5) nm and 84.3 (3.1) nm, respectively. The diabetic rats showed a significant decrease of GCX thickness in the retina, but not in the choroid, compared to controls (28.3 (0.3) nm, p<0.01 and 77.8 (1.4) nm, respectively). In the hypertensive rats, both retinal and choroidal GCX were significantly decreased compared to the control values (10.9 (0.4) nm and 13.2 (1.0) nm, respectively, both p<0.01). Moreover, we could visualize the adhesion of leukocytes and platelets on the luminal surface of VEC, at the site where the GCX was markedly degraded. These findings suggest that the GCX prevents adhesion of leukocytes and platelets to the VEC surface, and this impairment may lead to ocular vasculopathy in diabetes and hypertension.

Original languageEnglish
Pages (from-to)277-283
Number of pages7
JournalActa medica Okayama
Volume64
Issue number5
Publication statusPublished - 2010

Keywords

  • Choroid
  • Diabetes
  • Glycocalyx
  • Hypertension
  • Retina

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Glycocalyx degradation in retinal and choroidal capillary endothelium in rats with diabetes and hypertension'. Together they form a unique fingerprint.

Cite this