Glycocalyx damage estimated using colloidal iron staining

Daoxin Zhang, Yoshitaka Isaka, Ryoichi Imamura, Naotsugu Ichimaru, Yi Shi, Enyu Imai, Ye Tian, Aiji Ohtsuka, Shiro Takahara

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Anionic constituents in the peritubular capillary basement membranes and the glomerular endothelial cells have been demonstrated to function as a size- and charge-selective barrier. Cationic colloidal iron staining of human biopsy specimens revealed a glycocalyx on the surface of the glomerular basement membrane (GBM), peritubular capillary (PTC) endothelial cells, and brush border of the tubular epithelial cells of normal kidney. However, the glycocalyx was abolished in the PTC wall of C4d-positive acute humoral rejected kidney, and in the GBM as well as the PTC wall of a chronic, allograft, nephropathy kidney. In addition, cyclosporine eliminated the glycocalyx in the PTC wall, while treatment with heparin inhibited the elimination of the PTC glycocalyx. In conclusion, the glycocalyx on the surface of the GBM and PTC is an important component in the endothelial cell barrier.

Original languageEnglish
Pages (from-to)159-163
Number of pages5
JournalCell Transplantation
Volume17
Issue number1-2
Publication statusPublished - 2008

Fingerprint

Glycocalyx
Endothelial cells
Glomerular Basement Membrane
Iron
Staining and Labeling
Endothelial Cells
Kidney
Biopsy
Brushes
Microvilli
Cyclosporine
Allografts
Heparin
Basement Membrane
Epithelial Cells

Keywords

  • Chronic allograft nephropathy
  • Cyclosporine
  • Glycocalyx
  • Humoral rejection
  • Transplantation

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

Zhang, D., Isaka, Y., Imamura, R., Ichimaru, N., Shi, Y., Imai, E., ... Takahara, S. (2008). Glycocalyx damage estimated using colloidal iron staining. Cell Transplantation, 17(1-2), 159-163.

Glycocalyx damage estimated using colloidal iron staining. / Zhang, Daoxin; Isaka, Yoshitaka; Imamura, Ryoichi; Ichimaru, Naotsugu; Shi, Yi; Imai, Enyu; Tian, Ye; Ohtsuka, Aiji; Takahara, Shiro.

In: Cell Transplantation, Vol. 17, No. 1-2, 2008, p. 159-163.

Research output: Contribution to journalArticle

Zhang, D, Isaka, Y, Imamura, R, Ichimaru, N, Shi, Y, Imai, E, Tian, Y, Ohtsuka, A & Takahara, S 2008, 'Glycocalyx damage estimated using colloidal iron staining', Cell Transplantation, vol. 17, no. 1-2, pp. 159-163.
Zhang D, Isaka Y, Imamura R, Ichimaru N, Shi Y, Imai E et al. Glycocalyx damage estimated using colloidal iron staining. Cell Transplantation. 2008;17(1-2):159-163.
Zhang, Daoxin ; Isaka, Yoshitaka ; Imamura, Ryoichi ; Ichimaru, Naotsugu ; Shi, Yi ; Imai, Enyu ; Tian, Ye ; Ohtsuka, Aiji ; Takahara, Shiro. / Glycocalyx damage estimated using colloidal iron staining. In: Cell Transplantation. 2008 ; Vol. 17, No. 1-2. pp. 159-163.
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