Abstract
The presence of 1.0 mol/L glycinebetaine during isolation of D1/D2/Cytb559 reaction centre (RC) complexes from photosystem II (PSll) membrane fragments preserved the photochemical activity, monitored as the light-induced reduction of pheophytin and electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol. Glycinebetaine also protected the D1/D2/Cytb559 complexes against strong light-induced damage to the photochemical reactions and the irreversible bleaching of β-carotene and chlorophyll. The presence of glycinebetaine also enhanced thermotolerance of the D1/D2/Cytb559 complexes isolated in the presence of 1.0 mol/L betaine with an increase in the temperature for 50% inactivation from 29°C to 35°C. The results indicate an increased supra-molecular structural stability in the presence of glycinebetaine.
| Original language | English |
|---|---|
| Pages (from-to) | 41-49 |
| Number of pages | 9 |
| Journal | Journal of Plant Physiology |
| Volume | 160 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2003 |
| Externally published | Yes |
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Keywords
- D1/D2/Cytb559 complex
- Glycinebetaine
- Heat stress
- Photoinactivation
ASJC Scopus subject areas
- Physiology
- Agronomy and Crop Science
- Plant Science
Cite this
Glycinebetaine protects the D1/D2/Cytb559 complex of photosystem II against photo-induced and heat-induced inactivation. / Allakhverdiev, Suleyman; Hayashi, Hidenori; Nishiyama, Yoshitaka; Ivanov, Alexander G.; Aliev, Jalal A.; Klimov, Vyacheslav V.; Murata, Norio; Carpentier, Robert.
In: Journal of Plant Physiology, Vol. 160, No. 1, 01.01.2003, p. 41-49.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Glycinebetaine protects the D1/D2/Cytb559 complex of photosystem II against photo-induced and heat-induced inactivation
AU - Allakhverdiev, Suleyman
AU - Hayashi, Hidenori
AU - Nishiyama, Yoshitaka
AU - Ivanov, Alexander G.
AU - Aliev, Jalal A.
AU - Klimov, Vyacheslav V.
AU - Murata, Norio
AU - Carpentier, Robert
PY - 2003/1/1
Y1 - 2003/1/1
N2 - The presence of 1.0 mol/L glycinebetaine during isolation of D1/D2/Cytb559 reaction centre (RC) complexes from photosystem II (PSll) membrane fragments preserved the photochemical activity, monitored as the light-induced reduction of pheophytin and electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol. Glycinebetaine also protected the D1/D2/Cytb559 complexes against strong light-induced damage to the photochemical reactions and the irreversible bleaching of β-carotene and chlorophyll. The presence of glycinebetaine also enhanced thermotolerance of the D1/D2/Cytb559 complexes isolated in the presence of 1.0 mol/L betaine with an increase in the temperature for 50% inactivation from 29°C to 35°C. The results indicate an increased supra-molecular structural stability in the presence of glycinebetaine.
AB - The presence of 1.0 mol/L glycinebetaine during isolation of D1/D2/Cytb559 reaction centre (RC) complexes from photosystem II (PSll) membrane fragments preserved the photochemical activity, monitored as the light-induced reduction of pheophytin and electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol. Glycinebetaine also protected the D1/D2/Cytb559 complexes against strong light-induced damage to the photochemical reactions and the irreversible bleaching of β-carotene and chlorophyll. The presence of glycinebetaine also enhanced thermotolerance of the D1/D2/Cytb559 complexes isolated in the presence of 1.0 mol/L betaine with an increase in the temperature for 50% inactivation from 29°C to 35°C. The results indicate an increased supra-molecular structural stability in the presence of glycinebetaine.
KW - D1/D2/Cytb559 complex
KW - Glycinebetaine
KW - Heat stress
KW - Photoinactivation
UR - http://www.scopus.com/inward/record.url?scp=0346037257&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0346037257&partnerID=8YFLogxK
U2 - 10.1078/0176-1617-00845
DO - 10.1078/0176-1617-00845
M3 - Article
C2 - 12685044
AN - SCOPUS:0346037257
VL - 160
SP - 41
EP - 49
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
SN - 0176-1617
IS - 1
ER -