Glycinebetaine protects the D1/D2/Cytb559 complex of photosystem II against photo-induced and heat-induced inactivation

Suleyman I. Allakhverdiev, Hidenori Hayashi, Yoshitaka Nishiyama, Alexander G. Ivanov, Jalal A. Aliev, Vyacheslav V. Klimov, Norio Murata, Robert Carpentier

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

The presence of 1.0 mol/L glycinebetaine during isolation of D1/D2/Cytb559 reaction centre (RC) complexes from photosystem II (PSll) membrane fragments preserved the photochemical activity, monitored as the light-induced reduction of pheophytin and electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol. Glycinebetaine also protected the D1/D2/Cytb559 complexes against strong light-induced damage to the photochemical reactions and the irreversible bleaching of β-carotene and chlorophyll. The presence of glycinebetaine also enhanced thermotolerance of the D1/D2/Cytb559 complexes isolated in the presence of 1.0 mol/L betaine with an increase in the temperature for 50% inactivation from 29°C to 35°C. The results indicate an increased supra-molecular structural stability in the presence of glycinebetaine.

Original languageEnglish
Pages (from-to)41-49
Number of pages9
JournalJournal of Plant Physiology
Volume160
Issue number1
DOIs
Publication statusPublished - Jan 1 2003

Keywords

  • D1/D2/Cytb559 complex
  • Glycinebetaine
  • Heat stress
  • Photoinactivation

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Fingerprint Dive into the research topics of 'Glycinebetaine protects the D1/D2/Cytb559 complex of photosystem II against photo-induced and heat-induced inactivation'. Together they form a unique fingerprint.

  • Cite this

    Allakhverdiev, S. I., Hayashi, H., Nishiyama, Y., Ivanov, A. G., Aliev, J. A., Klimov, V. V., Murata, N., & Carpentier, R. (2003). Glycinebetaine protects the D1/D2/Cytb559 complex of photosystem II against photo-induced and heat-induced inactivation. Journal of Plant Physiology, 160(1), 41-49. https://doi.org/10.1078/0176-1617-00845