TY - JOUR
T1 - Glycinebetaine protects the D1/D2/Cytb559 complex of photosystem II against photo-induced and heat-induced inactivation
AU - Allakhverdiev, Suleyman I.
AU - Hayashi, Hidenori
AU - Nishiyama, Yoshitaka
AU - Ivanov, Alexander G.
AU - Aliev, Jalal A.
AU - Klimov, Vyacheslav V.
AU - Murata, Norio
AU - Carpentier, Robert
N1 - Funding Information:
Acknowledgements. This work was supported, in part, by a Grant-in-Aid for Specially Promoted Research (No. 08102011) from the Ministry of Education, Science and Culture, Japan; by the National Institute for Basic Biology Cooperative Research Program on the Stress Tolerance of Plants; by Natural Sciences and Engineering research Council of Canada (NSERC); and by the Russian Foundation for Basic Research (00-04-48287). The authors are grateful to Prof. P. Mohanty for his helpful discussion and critical reading of the manuscript.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - The presence of 1.0 mol/L glycinebetaine during isolation of D1/D2/Cytb559 reaction centre (RC) complexes from photosystem II (PSll) membrane fragments preserved the photochemical activity, monitored as the light-induced reduction of pheophytin and electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol. Glycinebetaine also protected the D1/D2/Cytb559 complexes against strong light-induced damage to the photochemical reactions and the irreversible bleaching of β-carotene and chlorophyll. The presence of glycinebetaine also enhanced thermotolerance of the D1/D2/Cytb559 complexes isolated in the presence of 1.0 mol/L betaine with an increase in the temperature for 50% inactivation from 29°C to 35°C. The results indicate an increased supra-molecular structural stability in the presence of glycinebetaine.
AB - The presence of 1.0 mol/L glycinebetaine during isolation of D1/D2/Cytb559 reaction centre (RC) complexes from photosystem II (PSll) membrane fragments preserved the photochemical activity, monitored as the light-induced reduction of pheophytin and electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol. Glycinebetaine also protected the D1/D2/Cytb559 complexes against strong light-induced damage to the photochemical reactions and the irreversible bleaching of β-carotene and chlorophyll. The presence of glycinebetaine also enhanced thermotolerance of the D1/D2/Cytb559 complexes isolated in the presence of 1.0 mol/L betaine with an increase in the temperature for 50% inactivation from 29°C to 35°C. The results indicate an increased supra-molecular structural stability in the presence of glycinebetaine.
KW - D1/D2/Cytb559 complex
KW - Glycinebetaine
KW - Heat stress
KW - Photoinactivation
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U2 - 10.1078/0176-1617-00845
DO - 10.1078/0176-1617-00845
M3 - Article
C2 - 12685044
AN - SCOPUS:0346037257
VL - 160
SP - 41
EP - 49
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
SN - 0176-1617
IS - 1
ER -