Abstract
The presence of 1.0 mol/L glycinebetaine during isolation of D1/D2/Cytb559 reaction centre (RC) complexes from photosystem II (PSll) membrane fragments preserved the photochemical activity, monitored as the light-induced reduction of pheophytin and electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol. Glycinebetaine also protected the D1/D2/Cytb559 complexes against strong light-induced damage to the photochemical reactions and the irreversible bleaching of β-carotene and chlorophyll. The presence of glycinebetaine also enhanced thermotolerance of the D1/D2/Cytb559 complexes isolated in the presence of 1.0 mol/L betaine with an increase in the temperature for 50% inactivation from 29°C to 35°C. The results indicate an increased supra-molecular structural stability in the presence of glycinebetaine.
Original language | English |
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Pages (from-to) | 41-49 |
Number of pages | 9 |
Journal | Journal of Plant Physiology |
Volume | 160 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 1 2003 |
Keywords
- D1/D2/Cytb559 complex
- Glycinebetaine
- Heat stress
- Photoinactivation
ASJC Scopus subject areas
- Physiology
- Agronomy and Crop Science
- Plant Science