Glycinebetaine protects the D1/D2/Cytb559 complex of photosystem II against photo-induced and heat-induced inactivation

Suleyman I. Allakhverdiev; Hidenori Hayashi; Yoshitaka Nishiyama; Alexander G. Ivanov; Jalal A. Aliev; Vyacheslav V. Klimov; Norio Murata; Robert Carpentier

doi:10.1078/0176-1617-00845

Glycinebetaine protects the D1/D2/Cytb559 complex of photosystem II against photo-induced and heat-induced inactivation

Suleyman I. Allakhverdiev, Hidenori Hayashi, Yoshitaka Nishiyama, Alexander G. Ivanov, Jalal A. Aliev, Vyacheslav V. Klimov, Norio Murata, Robert Carpentier

Research output: Contribution to journal › Article › peer-review

111 Citations (Scopus)

Abstract

The presence of 1.0 mol/L glycinebetaine during isolation of D1/D2/Cytb559 reaction centre (RC) complexes from photosystem II (PSll) membrane fragments preserved the photochemical activity, monitored as the light-induced reduction of pheophytin and electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol. Glycinebetaine also protected the D1/D2/Cytb559 complexes against strong light-induced damage to the photochemical reactions and the irreversible bleaching of β-carotene and chlorophyll. The presence of glycinebetaine also enhanced thermotolerance of the D1/D2/Cytb559 complexes isolated in the presence of 1.0 mol/L betaine with an increase in the temperature for 50% inactivation from 29°C to 35°C. The results indicate an increased supra-molecular structural stability in the presence of glycinebetaine.

Original language	English
Pages (from-to)	41-49
Number of pages	9
Journal	Journal of Plant Physiology
Volume	160
Issue number	1
DOIs	https://doi.org/10.1078/0176-1617-00845
Publication status	Published - Jan 1 2003

Keywords

D1/D2/Cytb559 complex
Glycinebetaine
Heat stress
Photoinactivation

ASJC Scopus subject areas

Physiology
Agronomy and Crop Science
Plant Science

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10.1078/0176-1617-00845

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title = "Glycinebetaine protects the D1/D2/Cytb559 complex of photosystem II against photo-induced and heat-induced inactivation",

abstract = "The presence of 1.0 mol/L glycinebetaine during isolation of D1/D2/Cytb559 reaction centre (RC) complexes from photosystem II (PSll) membrane fragments preserved the photochemical activity, monitored as the light-induced reduction of pheophytin and electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol. Glycinebetaine also protected the D1/D2/Cytb559 complexes against strong light-induced damage to the photochemical reactions and the irreversible bleaching of β-carotene and chlorophyll. The presence of glycinebetaine also enhanced thermotolerance of the D1/D2/Cytb559 complexes isolated in the presence of 1.0 mol/L betaine with an increase in the temperature for 50% inactivation from 29°C to 35°C. The results indicate an increased supra-molecular structural stability in the presence of glycinebetaine.",

keywords = "D1/D2/Cytb559 complex, Glycinebetaine, Heat stress, Photoinactivation",

author = "Allakhverdiev, {Suleyman I.} and Hidenori Hayashi and Yoshitaka Nishiyama and Ivanov, {Alexander G.} and Aliev, {Jalal A.} and Klimov, {Vyacheslav V.} and Norio Murata and Robert Carpentier",

note = "Funding Information: Acknowledgements. This work was supported, in part, by a Grant-in-Aid for Specially Promoted Research (No. 08102011) from the Ministry of Education, Science and Culture, Japan; by the National Institute for Basic Biology Cooperative Research Program on the Stress Tolerance of Plants; by Natural Sciences and Engineering research Council of Canada (NSERC); and by the Russian Foundation for Basic Research (00-04-48287). The authors are grateful to Prof. P. Mohanty for his helpful discussion and critical reading of the manuscript.",

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T1 - Glycinebetaine protects the D1/D2/Cytb559 complex of photosystem II against photo-induced and heat-induced inactivation

AU - Allakhverdiev, Suleyman I.

AU - Hayashi, Hidenori

AU - Nishiyama, Yoshitaka

AU - Ivanov, Alexander G.

AU - Aliev, Jalal A.

AU - Klimov, Vyacheslav V.

AU - Murata, Norio

AU - Carpentier, Robert

N1 - Funding Information: Acknowledgements. This work was supported, in part, by a Grant-in-Aid for Specially Promoted Research (No. 08102011) from the Ministry of Education, Science and Culture, Japan; by the National Institute for Basic Biology Cooperative Research Program on the Stress Tolerance of Plants; by Natural Sciences and Engineering research Council of Canada (NSERC); and by the Russian Foundation for Basic Research (00-04-48287). The authors are grateful to Prof. P. Mohanty for his helpful discussion and critical reading of the manuscript.

PY - 2003/1/1

Y1 - 2003/1/1

N2 - The presence of 1.0 mol/L glycinebetaine during isolation of D1/D2/Cytb559 reaction centre (RC) complexes from photosystem II (PSll) membrane fragments preserved the photochemical activity, monitored as the light-induced reduction of pheophytin and electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol. Glycinebetaine also protected the D1/D2/Cytb559 complexes against strong light-induced damage to the photochemical reactions and the irreversible bleaching of β-carotene and chlorophyll. The presence of glycinebetaine also enhanced thermotolerance of the D1/D2/Cytb559 complexes isolated in the presence of 1.0 mol/L betaine with an increase in the temperature for 50% inactivation from 29°C to 35°C. The results indicate an increased supra-molecular structural stability in the presence of glycinebetaine.

AB - The presence of 1.0 mol/L glycinebetaine during isolation of D1/D2/Cytb559 reaction centre (RC) complexes from photosystem II (PSll) membrane fragments preserved the photochemical activity, monitored as the light-induced reduction of pheophytin and electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol. Glycinebetaine also protected the D1/D2/Cytb559 complexes against strong light-induced damage to the photochemical reactions and the irreversible bleaching of β-carotene and chlorophyll. The presence of glycinebetaine also enhanced thermotolerance of the D1/D2/Cytb559 complexes isolated in the presence of 1.0 mol/L betaine with an increase in the temperature for 50% inactivation from 29°C to 35°C. The results indicate an increased supra-molecular structural stability in the presence of glycinebetaine.

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KW - Glycinebetaine

KW - Heat stress

KW - Photoinactivation

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Glycinebetaine protects the D1/D2/Cytb559 complex of photosystem II against photo-induced and heat-induced inactivation

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Keywords

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