Glycinebetaine protects the D1/D2/Cytb559 complex of photosystem II against photo-induced and heat-induced inactivation

Suleyman Allakhverdiev, Hidenori Hayashi, Yoshitaka Nishiyama, Alexander G. Ivanov, Jalal A. Aliev, Vyacheslav V. Klimov, Norio Murata, Robert Carpentier

Research output: Contribution to journalArticle

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Abstract

The presence of 1.0 mol/L glycinebetaine during isolation of D1/D2/Cytb559 reaction centre (RC) complexes from photosystem II (PSll) membrane fragments preserved the photochemical activity, monitored as the light-induced reduction of pheophytin and electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol. Glycinebetaine also protected the D1/D2/Cytb559 complexes against strong light-induced damage to the photochemical reactions and the irreversible bleaching of β-carotene and chlorophyll. The presence of glycinebetaine also enhanced thermotolerance of the D1/D2/Cytb559 complexes isolated in the presence of 1.0 mol/L betaine with an increase in the temperature for 50% inactivation from 29°C to 35°C. The results indicate an increased supra-molecular structural stability in the presence of glycinebetaine.

Original languageEnglish
Pages (from-to)41-49
Number of pages9
JournalJournal of Plant Physiology
Volume160
Issue number1
DOIs
Publication statusPublished - Jan 1 2003
Externally publishedYes

Fingerprint

Photosystem II Protein Complex
betaine
photosystem II
Diphenylcarbazide
inactivation
Hot Temperature
Pheophytins
2,6-Dichloroindophenol
heat
Light
Betaine
Carotenoids
Chlorophyll
Electron Transport
Temperature
Membranes
photochemical reactions
carotenes
bleaching
heat tolerance

Keywords

  • D1/D2/Cytb559 complex
  • Glycinebetaine
  • Heat stress
  • Photoinactivation

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Glycinebetaine protects the D1/D2/Cytb559 complex of photosystem II against photo-induced and heat-induced inactivation. / Allakhverdiev, Suleyman; Hayashi, Hidenori; Nishiyama, Yoshitaka; Ivanov, Alexander G.; Aliev, Jalal A.; Klimov, Vyacheslav V.; Murata, Norio; Carpentier, Robert.

In: Journal of Plant Physiology, Vol. 160, No. 1, 01.01.2003, p. 41-49.

Research output: Contribution to journalArticle

Allakhverdiev, Suleyman ; Hayashi, Hidenori ; Nishiyama, Yoshitaka ; Ivanov, Alexander G. ; Aliev, Jalal A. ; Klimov, Vyacheslav V. ; Murata, Norio ; Carpentier, Robert. / Glycinebetaine protects the D1/D2/Cytb559 complex of photosystem II against photo-induced and heat-induced inactivation. In: Journal of Plant Physiology. 2003 ; Vol. 160, No. 1. pp. 41-49.
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AB - The presence of 1.0 mol/L glycinebetaine during isolation of D1/D2/Cytb559 reaction centre (RC) complexes from photosystem II (PSll) membrane fragments preserved the photochemical activity, monitored as the light-induced reduction of pheophytin and electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol. Glycinebetaine also protected the D1/D2/Cytb559 complexes against strong light-induced damage to the photochemical reactions and the irreversible bleaching of β-carotene and chlorophyll. The presence of glycinebetaine also enhanced thermotolerance of the D1/D2/Cytb559 complexes isolated in the presence of 1.0 mol/L betaine with an increase in the temperature for 50% inactivation from 29°C to 35°C. The results indicate an increased supra-molecular structural stability in the presence of glycinebetaine.

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