Glycinebetaine protects the D1/D2/Cytb559 complex of photosystem II against photo-induced and heat-induced inactivation

Suleyman I. Allakhverdiev, Hidenori Hayashi, Yoshitaka Nishiyama, Alexander G. Ivanov, Jalal A. Aliev, Vyacheslav V. Klimov, Norio Murata, Robert Carpentier

    Research output: Contribution to journalArticlepeer-review

    100 Citations (Scopus)

    Abstract

    The presence of 1.0 mol/L glycinebetaine during isolation of D1/D2/Cytb559 reaction centre (RC) complexes from photosystem II (PSll) membrane fragments preserved the photochemical activity, monitored as the light-induced reduction of pheophytin and electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol. Glycinebetaine also protected the D1/D2/Cytb559 complexes against strong light-induced damage to the photochemical reactions and the irreversible bleaching of β-carotene and chlorophyll. The presence of glycinebetaine also enhanced thermotolerance of the D1/D2/Cytb559 complexes isolated in the presence of 1.0 mol/L betaine with an increase in the temperature for 50% inactivation from 29°C to 35°C. The results indicate an increased supra-molecular structural stability in the presence of glycinebetaine.

    Original languageEnglish
    Pages (from-to)41-49
    Number of pages9
    JournalJournal of Plant Physiology
    Volume160
    Issue number1
    DOIs
    Publication statusPublished - Jan 1 2003

    Keywords

    • D1/D2/Cytb559 complex
    • Glycinebetaine
    • Heat stress
    • Photoinactivation

    ASJC Scopus subject areas

    • Physiology
    • Agronomy and Crop Science
    • Plant Science

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