Glycinebetaine alleviates the inhibitory effect of moderate heat stress on the repair of photosystem II during photoinhibition

Suleyman Allakhverdiev, Dmitry A. Los, Prasanna Mohanty, Yoshitaka Nishiyama, Norio Murata

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Transformation with the bacterial gene codA for choline oxidase allows Synechococcus sp. PCC 7942 cells to accumulate glycinebetaine when choline is supplemented exogenously. First, we observed two types of protective effect of glycinebetaine against heat-induced inactivation of photosystem II (PSII) in darkness; the codA transgene shifted the temperature range of inactivation of the oxygen-evolving complex from 40-52 °C (with half inactivation at 46 °C) to 46-60 °C (with half inactivation at 54 °C) and that of the photochemical reaction center from 44-55 °C (with half inactivation at 51 °C) to 52-63 °C (with half inactivation at 58 °C). However, in light, PSII was more sensitive to heat stress; when moderate heat stress, such as 40 °C, was combined with light stress, PSII was rapidly inactivated, although these stresses, when applied separately, did not inactivate either the oxygen-evolving complex or the photochemical reaction center. Further our studies demonstrated that the moderate heat stress inhibited the repair of PSII during photoinhibition at the site of synthesis de novo of the D1 protein but did not accelerate the photodamage directly. The codA transgene and, thus, the accumulation of glycinebetaine alleviated such an inhibitory effect of moderate heat stress on the repair of PSII by accelerating the synthesis of the D1 protein. We propose a hypothetical scheme for the cyanobacterial photosynthesis that moderate heat stress inhibits the translation machinery and glycinebetaine protects it against the heat-induced inactivation.

Original languageEnglish
Pages (from-to)1363-1371
Number of pages9
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1767
Issue number12
DOIs
Publication statusPublished - Dec 1 2007
Externally publishedYes

Fingerprint

Photosystem II Protein Complex
Repair
Hot Temperature
Photochemical reactions
choline oxidase
Transgenes
Oxygen
Synechococcus
Light
Bacterial Genes
Photosynthesis
Darkness
Choline
Machinery
Proteins
Genes
Temperature

Keywords

  • codA gene
  • Glycinebetaine
  • Heat stress
  • Photoinhibition
  • Photosystem II
  • Protein synthesis
  • Repair

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Glycinebetaine alleviates the inhibitory effect of moderate heat stress on the repair of photosystem II during photoinhibition. / Allakhverdiev, Suleyman; Los, Dmitry A.; Mohanty, Prasanna; Nishiyama, Yoshitaka; Murata, Norio.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1767, No. 12, 01.12.2007, p. 1363-1371.

Research output: Contribution to journalArticle

Allakhverdiev, Suleyman ; Los, Dmitry A. ; Mohanty, Prasanna ; Nishiyama, Yoshitaka ; Murata, Norio. / Glycinebetaine alleviates the inhibitory effect of moderate heat stress on the repair of photosystem II during photoinhibition. In: Biochimica et Biophysica Acta - Bioenergetics. 2007 ; Vol. 1767, No. 12. pp. 1363-1371.
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