セルロモナスNT3060株の防腐剤耐性グリセロールキナーゼの遺伝子クローニングと特性解析

Translated title of the contribution: Glycerol kinase from Cellulomonas sp. NT3060 which has high resistance against preservatives

曽我部 敦, 北林 雅夫, 森島 賢一, 古川 美代子, 八田 貴, 福田 靖久, 西瀬 弘, 岡 正則, Takashi Tamura, Kenji Inagaki

Research output: Contribution to journalArticle

Abstract

Glycerol kinase is suitable for clinical diagnostic use in levels of neutral fat. Glycerol kinase from Cellulomonas sp. NT3060 shows good stability against several preservatives such as N-methylisothiazolone and imidazolidinylurea. We have cloned glpK gene from Cellulomonas sp. NT3060. This glpK gene is composed of 1,518 bp nucleotides and encodes 505 amino acid residues. The glpK gene was expressed in Escherichia coli and Cellulomonas sp. glycerol kinase was purified to homogeneity by column chromatography. The glycerol kinase exhibited high tolerance to various preservatives compared to other glycerol kinases. This tolerance was found to be favorable feature as diagnostic reagent for quantity of triglyceride.
Original languageJapanese
Pages (from-to)402-409
Number of pages8
JournalSeibutsu-kogaku Kaishi
Volume92
Issue number8
Publication statusPublished - Aug 25 2014

Fingerprint

glycerol kinase
Cellulomonas
Glycerol Kinase
preservatives
triacylglycerols
Genes
genes
Chromatography
chromatography
Triglycerides
Nucleotides
nucleotides
Fats
Escherichia coli
Amino Acids
amino acids

Keywords

  • glycerol kinase
  • triglyceride measurement
  • preservative tolerance
  • Cellulomonas

Cite this

曽我部敦, 北林雅夫, 森島賢一, 古川美代子, 八田貴, 福田靖久, ... Inagaki, K. (2014). セルロモナスNT3060株の防腐剤耐性グリセロールキナーゼの遺伝子クローニングと特性解析. Seibutsu-kogaku Kaishi, 92(8), 402-409.

セルロモナスNT3060株の防腐剤耐性グリセロールキナーゼの遺伝子クローニングと特性解析. / 曽我部敦; 北林雅夫; 森島賢一; 古川美代子; 八田貴; 福田靖久; 西瀬弘; 岡正則; Tamura, Takashi; Inagaki, Kenji.

In: Seibutsu-kogaku Kaishi, Vol. 92, No. 8, 25.08.2014, p. 402-409.

Research output: Contribution to journalArticle

曽我部敦, 北林雅夫, 森島賢一, 古川美代子, 八田貴, 福田靖久, 西瀬弘, 岡正則, Tamura, T & Inagaki, K 2014, 'セルロモナスNT3060株の防腐剤耐性グリセロールキナーゼの遺伝子クローニングと特性解析', Seibutsu-kogaku Kaishi, vol. 92, no. 8, pp. 402-409.
曽我部敦, 北林雅夫, 森島賢一, 古川美代子, 八田貴, 福田靖久 et al. セルロモナスNT3060株の防腐剤耐性グリセロールキナーゼの遺伝子クローニングと特性解析. Seibutsu-kogaku Kaishi. 2014 Aug 25;92(8):402-409.
曽我部敦 ; 北林雅夫 ; 森島賢一 ; 古川美代子 ; 八田貴 ; 福田靖久 ; 西瀬弘 ; 岡正則 ; Tamura, Takashi ; Inagaki, Kenji. / セルロモナスNT3060株の防腐剤耐性グリセロールキナーゼの遺伝子クローニングと特性解析. In: Seibutsu-kogaku Kaishi. 2014 ; Vol. 92, No. 8. pp. 402-409.
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