Glycerol kinase from Cellulomonas sp. NT3060 which has high resistance against preservatives

Atsushi Sogabe, Masao Kitabayashi, Kenichi Morishima, Miyoko Furukawa, Takashi Hatta, Yasuhisa Fukuda, Hiroshi Nishise, Masanori Oka, Takashi Tamura, Kenji Inagaki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Glycerol kinase is suitable for clinical diagnostic use in levels of neutral fat. Glycerol kinase from Cellulomonas sp. NT3060 shows good stability against several preservatives such as N-methylisothiazolone and imidazolidinylurea. We have cloned glpK gene from Cellulomonas sp. NT3060. This glpK gene is composed of 1,518 bp nucleotides and encodes 505 amino acid residues. The glpK gene was expressed in Escherichia coli and Cellulomonas sp. glycerol kinase was purified to homogeneity by column chromatog-raphy. The glycerol kinase exhibited high tolerance to various preservatives compared to other glycerol kinases. This tolerance was found to be favorable feature as diagnostic reagent for quantity of triglyceride.

Original languageEnglish
Pages (from-to)402-409
Number of pages8
JournalSeibutsu-kogaku Kaishi
Volume92
Issue number8
Publication statusPublished - Jan 1 2014

Keywords

  • Glycerol kinase
  • Preservative tolerance
  • Triglyceride measurement

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology

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  • Cite this

    Sogabe, A., Kitabayashi, M., Morishima, K., Furukawa, M., Hatta, T., Fukuda, Y., Nishise, H., Oka, M., Tamura, T., & Inagaki, K. (2014). Glycerol kinase from Cellulomonas sp. NT3060 which has high resistance against preservatives. Seibutsu-kogaku Kaishi, 92(8), 402-409.