Glutathione transport systems of the budding yeast saccharomyces cerevisiae

Tsuyoshi Miyake, Toshiya Hazu, Sayaka Yoshida, Munetoshi Kanayama, Ken Ichi Tomochika, Sumio Shinoda, Bun Ichiro Ono

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The budding yeast Saccharomyces cerevisiae was shown to have two kinetically distinguishable glutathione transport systems. While one with high affinity (GSH-P1; KT=0.045 mM) was regulated, the other with low affinity (GSH-P2; KT>2 mM) was not. GSH-P1 was highly specific to glutathione, and its activity was quickly lost by suspending the cells in buffer solutions. This activity loss was not observed if glucose-containing buffer was used. In addition, ρ isolates had only about one half of the glutathione transport activity of the original (ρ +) strain. Therefore, it is concluded that GSH-P1 is an ATP-driven transport system. Strong and moderate inhibition of GSH-P1 by protonophores and ionophores, respectively, are attributed to competition for ATP between GSH-P1 and proton- and cation-pumps, re.

Original languageEnglish
Pages (from-to)1858-1864
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number10
DOIs
Publication statusPublished - Jan 1 1998

Keywords

  • Glutathione
  • Inhibition
  • Kinetic analysis
  • Saccharomyces cerevisiae
  • Transport

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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  • Cite this

    Miyake, T., Hazu, T., Yoshida, S., Kanayama, M., Tomochika, K. I., Shinoda, S., & Ono, B. I. (1998). Glutathione transport systems of the budding yeast saccharomyces cerevisiae. Bioscience, Biotechnology and Biochemistry, 62(10), 1858-1864. https://doi.org/10.1271/bbb.62.1858