Glutathione Transport Systems of the Budding Yeast Saccharomyces cerevisiae

Tsuyoshi Miyake, Toshiya Hazu, Sayaka Yoshida, Munetoshi Kanayama, Ken Ichi Tomochika, Sumio Shinoda, Bun Ichiro Ono

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The budding yeast Saccharomyces cerevisiae was shown to have two kinetically distinguishable glutathione transport systems. While one with high affinity (GSH-P1; KT =0.045 mM) was regulated, the other with low affinity (GSH-P2; KT>2 mM) was not. GSH-P1 was highly specific to glutathione, and its activity was quickly lost by suspending the cells in buffer solutions. This activity loss was not observed if glucose-containing buffer was used. In addition, ρ- isolates had only about one half of the glutathione transport activity of the original (ρ+) strain. Therefore, it is concluded that GSH-P1 is an ATP-driven transport system. Strong and moderate inhibition of GSH-P1 by protonophores and ionophores, respectively, are attributed to competition for ATP between GSH-P1 and proton- and cation-pumps, respectively.

Original languageEnglish
Pages (from-to)1858-1864
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Issue number10
Publication statusPublished - Oct 1998



  • Glutathione
  • Inhibition
  • Kinetic analysis
  • Saccharomyces cerevisiae
  • Transport

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Miyake, T., Hazu, T., Yoshida, S., Kanayama, M., Tomochika, K. I., Shinoda, S., & Ono, B. I. (1998). Glutathione Transport Systems of the Budding Yeast Saccharomyces cerevisiae. Bioscience, Biotechnology and Biochemistry, 62(10), 1858-1864.