Silica gel and glass beads were modified by using acid chloride of metal-tetrakis(4-carboxyphenyl)porphine (M-TCPP) through a peptide bond, and an anion-exchange resin with M-TCPP by ion-exchange reaction and physical adsorption. The carriers modified with Co3+-TCPP proved to accelerate the redox reaction which is catalyzed by glutathione oxidase (GSHOx), while those modified with Mn3+ -TCPP exhibited no activity. Formation of GS-SG and hydrogen peroxide was confirmed by means of mass spectroscopy and colored reaction, respectively. The silica gel modified with Co3+ -TCPP exhibited the strongest activity among the tested carriers, and was expected to be useful practically as a solid catalyst for the determination of glutathione.
- Carriers modified with Co - tetra-(4-carboxyphenyl)porphine
- Glutathione oxidase-like activity
- Hydrogen peroxide
- Oxidation of glutathione
ASJC Scopus subject areas
- Analytical Chemistry