TY - JOUR
T1 - Glutathione oxidase-like activity of glass beads, silica gel and anion-exchange resin modified with cobalt(III)- tetrakis(4,carboxyphenyl)porphine
AU - Mifune, Masaki
AU - Harada, Rumiko
AU - Iwado, Akimasa
AU - Motohashi, Noriko
AU - Saito, Yutaka
N1 - Funding Information:
The authors thank Miss Chizuko Kodama for her technical support of the measurement of the mass spectra. This work was supported in part by a Grant-in-Aid for Scientific Research from Ministry of Education, Science and Culture, Japan.
PY - 1998/8
Y1 - 1998/8
N2 - Silica gel and glass beads were modified by using acid chloride of metal-tetrakis(4-carboxyphenyl)porphine (M-TCPP) through a peptide bond, and an anion-exchange resin with M-TCPP by ion-exchange reaction and physical adsorption. The carriers modified with Co3+-TCPP proved to accelerate the redox reaction which is catalyzed by glutathione oxidase (GSHOx), while those modified with Mn3+ -TCPP exhibited no activity. Formation of GS-SG and hydrogen peroxide was confirmed by means of mass spectroscopy and colored reaction, respectively. The silica gel modified with Co3+ -TCPP exhibited the strongest activity among the tested carriers, and was expected to be useful practically as a solid catalyst for the determination of glutathione.
AB - Silica gel and glass beads were modified by using acid chloride of metal-tetrakis(4-carboxyphenyl)porphine (M-TCPP) through a peptide bond, and an anion-exchange resin with M-TCPP by ion-exchange reaction and physical adsorption. The carriers modified with Co3+-TCPP proved to accelerate the redox reaction which is catalyzed by glutathione oxidase (GSHOx), while those modified with Mn3+ -TCPP exhibited no activity. Formation of GS-SG and hydrogen peroxide was confirmed by means of mass spectroscopy and colored reaction, respectively. The silica gel modified with Co3+ -TCPP exhibited the strongest activity among the tested carriers, and was expected to be useful practically as a solid catalyst for the determination of glutathione.
KW - Carriers modified with Co - tetra-(4-carboxyphenyl)porphine
KW - Glutathione oxidase-like activity
KW - Hydrogen peroxide
KW - Oxidation of glutathione
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U2 - 10.1016/S0039-9140(98)00042-3
DO - 10.1016/S0039-9140(98)00042-3
M3 - Article
C2 - 18967291
AN - SCOPUS:0032146523
SN - 0039-9140
VL - 46
SP - 1583
EP - 1590
JO - Talanta
JF - Talanta
IS - 6
ER -