Glutathione oxidase-like activity of glass beads, silica gel and anion-exchange resin modified with cobalt(III)- tetrakis(4,carboxyphenyl)porphine

Masaki Mifune, Rumiko Harada, Akimasa Iwado, Noriko Motohashi, Yutaka Saito

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Silica gel and glass beads were modified by using acid chloride of metal-tetrakis(4-carboxyphenyl)porphine (M-TCPP) through a peptide bond, and an anion-exchange resin with M-TCPP by ion-exchange reaction and physical adsorption. The carriers modified with Co3+-TCPP proved to accelerate the redox reaction which is catalyzed by glutathione oxidase (GSHOx), while those modified with Mn3+ -TCPP exhibited no activity. Formation of GS-SG and hydrogen peroxide was confirmed by means of mass spectroscopy and colored reaction, respectively. The silica gel modified with Co3+ -TCPP exhibited the strongest activity among the tested carriers, and was expected to be useful practically as a solid catalyst for the determination of glutathione.

Original languageEnglish
Pages (from-to)1583-1590
Number of pages8
JournalTalanta
Volume46
Issue number6
DOIs
Publication statusPublished - Aug 1998

Keywords

  • Carriers modified with Co - tetra-(4-carboxyphenyl)porphine
  • Glutathione oxidase-like activity
  • Hydrogen peroxide
  • Oxidation of glutathione

ASJC Scopus subject areas

  • Analytical Chemistry

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