Glutamine is a key regulator for amino acid-controlled cell growth through the mTOR signaling pathway in rat intestinal epithelial cells

Tohru Nakajo, Tomoki Yamatsuji, Hidetoshi Ban, Kaori Shigemitsu, Minoru Haisa, Takayuki Motoki, Kazuhiro Noma, Tetsuji Nobuhisa, Junji Matsuoka, Mehmet Gunduz, Kazuyoshi Yonezawa, Noriaki Tanaka, Yoshio Naomoto

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Amino acids, especially branched-chain amino acids such as l-leucine, have been shown to regulate activation of p70 S6 kinase and phosphorylation of 4E-BP1 through the mTOR signaling pathway. In our recent study, l-arginine was also shown to activate the mTOR signaling pathway in rat intestinal epithelial cells. l-Glutamine is an amino acid that is required for culturing of numerous cell types, including rat intestinal epithelial cells. In this study, we showed that l-glutamine inhibited the activation of p70 S6 kinase and phosphorylation of 4E-BP1 induced by arginine or leucine in rat intestinal epithelial cells. Although the molecular mechanism of l-glutamine-induced inhibition of the mTOR signaling pathway is still unknown, the presence of this novel signal pathway may indicate that individual amino acids play specific roles for cellular proliferation and growth.

Original languageEnglish
Pages (from-to)174-180
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume326
Issue number1
DOIs
Publication statusPublished - Dec 31 2004
Externally publishedYes

Fingerprint

Cell growth
Glutamine
70-kDa Ribosomal Protein S6 Kinases
Rats
Phosphorylation
Epithelial Cells
Amino Acids
Leucine
Arginine
Growth
Chemical activation
Branched Chain Amino Acids
Amino acids
Signal Transduction
Cell Proliferation

Keywords

  • 4E-BP1
  • IEC16
  • IEC18
  • l-Arginine
  • l-Glutamine
  • l-Leucine
  • mTOR
  • p70 S6 kinase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Glutamine is a key regulator for amino acid-controlled cell growth through the mTOR signaling pathway in rat intestinal epithelial cells. / Nakajo, Tohru; Yamatsuji, Tomoki; Ban, Hidetoshi; Shigemitsu, Kaori; Haisa, Minoru; Motoki, Takayuki; Noma, Kazuhiro; Nobuhisa, Tetsuji; Matsuoka, Junji; Gunduz, Mehmet; Yonezawa, Kazuyoshi; Tanaka, Noriaki; Naomoto, Yoshio.

In: Biochemical and Biophysical Research Communications, Vol. 326, No. 1, 31.12.2004, p. 174-180.

Research output: Contribution to journalArticle

Nakajo, T, Yamatsuji, T, Ban, H, Shigemitsu, K, Haisa, M, Motoki, T, Noma, K, Nobuhisa, T, Matsuoka, J, Gunduz, M, Yonezawa, K, Tanaka, N & Naomoto, Y 2004, 'Glutamine is a key regulator for amino acid-controlled cell growth through the mTOR signaling pathway in rat intestinal epithelial cells', Biochemical and Biophysical Research Communications, vol. 326, no. 1, pp. 174-180. https://doi.org/10.1016/j.bbrc.2004.11.015
Nakajo, Tohru ; Yamatsuji, Tomoki ; Ban, Hidetoshi ; Shigemitsu, Kaori ; Haisa, Minoru ; Motoki, Takayuki ; Noma, Kazuhiro ; Nobuhisa, Tetsuji ; Matsuoka, Junji ; Gunduz, Mehmet ; Yonezawa, Kazuyoshi ; Tanaka, Noriaki ; Naomoto, Yoshio. / Glutamine is a key regulator for amino acid-controlled cell growth through the mTOR signaling pathway in rat intestinal epithelial cells. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 326, No. 1. pp. 174-180.
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