Glutamate functions in stomatal closure in Arabidopsis and fava bean

Riichiro Yoshida, Izumi Mori, Nobuto Kamizono, Yudai Shichiri, Tetsuo Shimatani, Fumika Miyata, Kenji Honda, Sumio Iwai

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Guard cells are indispensable for higher plants because they control gas exchange and water balance to maintain photosynthetic activity. The signaling processes that govern their movement are controlled by several factors, such as abscisic acid (ABA), blue light, pathogen-associated molecular patterns (PAMPs), and carbon dioxide. Herein, we demonstrated that the amino acid glutamate (Glu), a well-known mammalian neurotransmitter, functions as a novel signaling molecule in stomatal closure in both Arabidopsis and fava bean (Vicia faba L.). Pharmacological and electrophysiological analyses provided important clues for the participation of Glu-receptors, Ca2+, and protein phosphorylation during the signaling process. Genetic analyses using Arabidopsis ABA-deficient (aba2-1) and ABA-insensitive (abi1-1 and abi2-1) mutants showed that ABA is not required for Glu signaling. However, loss-of-function of the Arabidopsis gene encoding Slow Anion Channel-Associated1 (SLAC1) and Calcium-Dependent Protein Kinase 6 (CPK6) impaired the Glu response. Moreover, T-DNA knockout mutations of the Arabidopsis Glu receptor-like gene (GLR), GLR3.5, lost their sensitivity to Glu-dependent stomatal closure. Our results strongly support functional Glu-signaling in stomatal closure and the crucial roles of GLRs in this signaling process.

Original languageEnglish
Pages (from-to)39-49
Number of pages11
JournalJournal of Plant Research
Volume129
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

Keywords

  • Arabidopis thaliana
  • Glutamate
  • Glutamate receptor
  • Signal transduction
  • Stomatal closure
  • Vicia faba

ASJC Scopus subject areas

  • Plant Science

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  • Cite this

    Yoshida, R., Mori, I., Kamizono, N., Shichiri, Y., Shimatani, T., Miyata, F., Honda, K., & Iwai, S. (2016). Glutamate functions in stomatal closure in Arabidopsis and fava bean. Journal of Plant Research, 129(1), 39-49. https://doi.org/10.1007/s10265-015-0757-0