Glutamate enhances caspase-3 immunoreactivity in cultured spinal cord neurons of newborn rats

Y. Manabe, J. M. Wang, M. Shiote, T. Murakami, I. Nagano, M. Shoji, Koji Abe

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The role of glutamate in the mechanism of spinal neuron death is not fully understood. With addition of glutamate to primary culture of 11-day-old rat spinal cord, the number of caspase-3 positive small neurons of the dorsal horn greatly increased at 6-24 h in contrast to the case with vehicle. The addition of glutamate made caspase-3 immunoreactivity stronger in the cytoplasm of large motor neurons in the ventral horn. The present results show that excessive amount of glutamate enhances apoptotic pathway through caspase-3 in cultured spinal neurons of newborn rat.

Original languageEnglish
Pages (from-to)312-316
Number of pages5
JournalNeurological Research
Volume25
Issue number3
DOIs
Publication statusPublished - Apr 1 2003

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Keywords

  • Caspase-3
  • Glutamate
  • Newborn rat
  • Spinal cord neurons

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

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