TY - JOUR
T1 - Glucosinolate degradation products, isothiocyanates, nitriles, and thiocyanates, induce stomatal closure accompanied by peroxidase-mediated reactive oxygen species production in arabidopsis thaliana
AU - Hossain, Mohammad Shakhawat
AU - Ye, Wenxiu
AU - Hossain, Mohammad Anowar
AU - Okuma, Eiji
AU - Uraji, Misugi
AU - Nakamura, Yoshimasa
AU - Mori, Izumi C.
AU - Murata, Yoshiyuki
N1 - Funding Information:
This research was supported in part by Grants for Scientific Research in Priority Areas from the Ministry of Education, Culture, Sports, and Science and Technology of Japan.
PY - 2013
Y1 - 2013
N2 - Isothiocyanates, nitriles, and thiocyanates are degradation products of glucosinolates in crucifer plants. In this study, we investigated the stomatal response to allyl isothiocyanate (AITC), 3-butenenitrile (3BN), and ethyl thiocyanate (ESCN) in Arabidopsis. AITC, 3BN, and ESCN induced stomatal closure in the wild type and the atrbohD atrbohF mutant. Stomatal closure was inhibited by catalase and salicylhydroxamic acid (SHAM). The degradation products induced extracellular reactive oxygen species (ROS) production in the rosette leaves, and intracellular ROS accumulation, NO production, and cytosolic free calcium concentration ([Ca2+]cyt) oscillations in guard cells, which were inhibited by SHAM. These results suggest that glucosinolate degradation products induce stomatal closure accompanied by extracellular ROS production mediated by SHAMsensitive peroxidases, intracellular ROS accumulation, and [Ca 2+]cyt oscillation in Arabidopsis.
AB - Isothiocyanates, nitriles, and thiocyanates are degradation products of glucosinolates in crucifer plants. In this study, we investigated the stomatal response to allyl isothiocyanate (AITC), 3-butenenitrile (3BN), and ethyl thiocyanate (ESCN) in Arabidopsis. AITC, 3BN, and ESCN induced stomatal closure in the wild type and the atrbohD atrbohF mutant. Stomatal closure was inhibited by catalase and salicylhydroxamic acid (SHAM). The degradation products induced extracellular reactive oxygen species (ROS) production in the rosette leaves, and intracellular ROS accumulation, NO production, and cytosolic free calcium concentration ([Ca2+]cyt) oscillations in guard cells, which were inhibited by SHAM. These results suggest that glucosinolate degradation products induce stomatal closure accompanied by extracellular ROS production mediated by SHAMsensitive peroxidases, intracellular ROS accumulation, and [Ca 2+]cyt oscillation in Arabidopsis.
KW - 3-butenenitrile
KW - Allyl isothiocyanate
KW - Ethyl thiocyanate
KW - Glucosinolates
KW - Reactive oxygen species
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U2 - 10.1271/bbb.120928
DO - 10.1271/bbb.120928
M3 - Article
C2 - 23649257
AN - SCOPUS:84878729768
SN - 0916-8451
VL - 77
SP - 977
EP - 983
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 5
ER -