Glucose utilization in the brain during acute seizure is a useful biomarker for the evaluation of anticonvulsants: effect of methyl ethyl ketone in lithium-pilocarpine status epilepticus rats

Akifumi Yamada, Sotaro Momosaki, Rie Hosoi, Koji Abe, Masatoshi Yamaguchi, Osamu Inoue

Research output: Contribution to journalArticle

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Abstract

Enhancement of glucose utilization in the brain has been well known during acute seizure in various kinds of animal model of epilepsy. This enhancement of glucose utilization might be related to neural damage in these animal models. Recently, we found that methyl ethyl ketone (MEK) had both anticonvulsive and neuroprotective effects in lithium-pilocapine (Li-pilo) status epilepticus (SE) rat. In this article, we measured the uptake of [14C]2-deoxyglucose ([14C]DG) in the Li-pilo SE and Li-pilo SE with MEK rat brain in order to assess whether the glucose utilization was a useful biomarker for the detection of efficacy of anticonvulsive compounds. Significant increase of [14C]DG uptake (45 min after the injection) in the cerebral cortex, hippocampus, amygdala and thalamus during acute seizure induced by Li-pilo were observed. On the other hand, the initial uptake of [14C]DG (1 min after the injection) in the Li-pilo SE rats was not different from the control rats. Therefore, the enhancement of glucose metabolism during acute seizure was due to the facilitation of the rate of phosphorylation process of [14C]DG in the brain. Pretreatment with MEK (8 mmol/kg) completely abolished the enhancement of glucose utilization in the Li-pilo SE rats. The present results indicated that glucose utilization in the brain during acute seizure might be a useful biomarker for the evaluation of efficacy of anticonvulsive compounds.

Original languageEnglish
Pages (from-to)949-954
Number of pages6
JournalNuclear Medicine and Biology
Volume36
Issue number8
DOIs
Publication statusPublished - Nov 2009
Externally publishedYes

Fingerprint

Pilocarpine
Status Epilepticus
Lithium
Anticonvulsants
Seizures
Biomarkers
Glucose
Brain
Animal Models
Injections
Deoxyglucose
Neuroprotective Agents
Amygdala
Thalamus
Cerebral Cortex
methylethyl ketone
Epilepsy
Hippocampus
Phosphorylation

Keywords

  • [C]DG
  • Anticonvulsant
  • Glucose utilization
  • Lithium-pilocarpine
  • Methyl ethyl ketone
  • Seizure

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging

Cite this

Glucose utilization in the brain during acute seizure is a useful biomarker for the evaluation of anticonvulsants : effect of methyl ethyl ketone in lithium-pilocarpine status epilepticus rats. / Yamada, Akifumi; Momosaki, Sotaro; Hosoi, Rie; Abe, Koji; Yamaguchi, Masatoshi; Inoue, Osamu.

In: Nuclear Medicine and Biology, Vol. 36, No. 8, 11.2009, p. 949-954.

Research output: Contribution to journalArticle

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