Abstract
Three experiments were conducted in which 2-cell bovine embryos were prepared from oocytes, obtained from abattoir ovaries, by in-vitro maturation for 22 to 24 hours, followed by exposure to spermatozoa for 8 hours and culture for 40 hours within the cumulus. The cumulus cells were then removed, and the cleaved embryos were cultured for a further 120 hours or longer, in the presence or absence of glucose, pyruvate and lactate. Very few embryos developed in the complete absence of energy substrates. Lactate and pyruvate, alone or combined, supported development to the 8-cell stage, but pyruvate was required to support development to the morula stage (Experiment 1). When present throughout culture or when added at 48 or 96 hours postinsemination, 5.56 mM glucose was detrimental to development (Experiments 1 and 2). However, when added at 120 hours postinsemination, 5.56 mM glucose improved development to the blastocyst and expanded blastocyst stages, compared with no glucose or 11.12 mM glucose (Experiment 3).
| Original language | English |
|---|---|
| Pages (from-to) | 875-886 |
| Number of pages | 12 |
| Journal | Theriogenology |
| Volume | 39 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1993 |
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Keywords
- bovine
- embryo development
- energy substrates
- in vitro fertilization
- oocytes
ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)
Cite this
Glucose requirement at different developmental stages of in vitro fertilized bovine embryos cultured in semi-defined medium. / Kim, J. H.; Funahashi, Hiroaki; Niwa, K.; Okuda, K.
In: Theriogenology, Vol. 39, No. 4, 1993, p. 875-886.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Glucose requirement at different developmental stages of in vitro fertilized bovine embryos cultured in semi-defined medium
AU - Kim, J. H.
AU - Funahashi, Hiroaki
AU - Niwa, K.
AU - Okuda, K.
PY - 1993
Y1 - 1993
N2 - Three experiments were conducted in which 2-cell bovine embryos were prepared from oocytes, obtained from abattoir ovaries, by in-vitro maturation for 22 to 24 hours, followed by exposure to spermatozoa for 8 hours and culture for 40 hours within the cumulus. The cumulus cells were then removed, and the cleaved embryos were cultured for a further 120 hours or longer, in the presence or absence of glucose, pyruvate and lactate. Very few embryos developed in the complete absence of energy substrates. Lactate and pyruvate, alone or combined, supported development to the 8-cell stage, but pyruvate was required to support development to the morula stage (Experiment 1). When present throughout culture or when added at 48 or 96 hours postinsemination, 5.56 mM glucose was detrimental to development (Experiments 1 and 2). However, when added at 120 hours postinsemination, 5.56 mM glucose improved development to the blastocyst and expanded blastocyst stages, compared with no glucose or 11.12 mM glucose (Experiment 3).
AB - Three experiments were conducted in which 2-cell bovine embryos were prepared from oocytes, obtained from abattoir ovaries, by in-vitro maturation for 22 to 24 hours, followed by exposure to spermatozoa for 8 hours and culture for 40 hours within the cumulus. The cumulus cells were then removed, and the cleaved embryos were cultured for a further 120 hours or longer, in the presence or absence of glucose, pyruvate and lactate. Very few embryos developed in the complete absence of energy substrates. Lactate and pyruvate, alone or combined, supported development to the 8-cell stage, but pyruvate was required to support development to the morula stage (Experiment 1). When present throughout culture or when added at 48 or 96 hours postinsemination, 5.56 mM glucose was detrimental to development (Experiments 1 and 2). However, when added at 120 hours postinsemination, 5.56 mM glucose improved development to the blastocyst and expanded blastocyst stages, compared with no glucose or 11.12 mM glucose (Experiment 3).
KW - bovine
KW - embryo development
KW - energy substrates
KW - in vitro fertilization
KW - oocytes
UR - http://www.scopus.com/inward/record.url?scp=0000892765&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0000892765&partnerID=8YFLogxK
U2 - 10.1016/0093-691X(93)90425-5
DO - 10.1016/0093-691X(93)90425-5
M3 - Article
AN - SCOPUS:0000892765
VL - 39
SP - 875
EP - 886
JO - Theriogenology
JF - Theriogenology
SN - 0093-691X
IS - 4
ER -