Glucose modulates the release of histamine from the mouse hypothalamus in vitro

Masahiro Nishibori, R. Oishi, Y. Itoh, K. Saeki

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The effect of glucose concentration on the in vitro release of histamine (HA) was examined, using two different preparations of the mouse hypothalamus. The HA and tele-methylhistamine released from whole blocks of the hypothalamus into the medium linearly increased during 2-h incubation in normal Krebs-Ringer bicarbonate solution in the absence of external depolarizing stimuli. The release of HA from this preparation depended on the temperature and Ca2+ in the medium and was progressively increased with decrease in the glucose concentration from 11.5 to 1 mM. The rate of the HA release was dependent on the absolute concentration of glucose and not on an abrupt change in the concentration. When slices of the hypothalamus were incubated in high K+ medium, a temperature- and Ca2+-dependent HA release was observed. At low concentrations of glucose, the K+ (20 mM)-induced HA release from the hypothalamic slices was also enhanced. Tetrodotoxin (10 μm) inhibited the enhancing effect of a low glucose concentration (2 mM) on the HA release by 60%, in both preparations of the hypothalamus. The possibility that the release of HA from the mouse hypothalamus is regulated by glucose concentration and that activation of neuronal Na+ channels is involved in the enhancement of the HA release by low glucose concentrations warrants further attention.

Original languageEnglish
Pages (from-to)1761-1767
Number of pages7
JournalJournal of Neurochemistry
Volume47
Issue number6
Publication statusPublished - 1986

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Histamine Release
Histamine
Hypothalamus
Glucose
Temperature
In Vitro Techniques
Tetrodotoxin
Bicarbonates
Chemical activation

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Glucose modulates the release of histamine from the mouse hypothalamus in vitro. / Nishibori, Masahiro; Oishi, R.; Itoh, Y.; Saeki, K.

In: Journal of Neurochemistry, Vol. 47, No. 6, 1986, p. 1761-1767.

Research output: Contribution to journalArticle

Nishibori, Masahiro ; Oishi, R. ; Itoh, Y. ; Saeki, K. / Glucose modulates the release of histamine from the mouse hypothalamus in vitro. In: Journal of Neurochemistry. 1986 ; Vol. 47, No. 6. pp. 1761-1767.
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